Grafting versus seed propagated apricot populations: two main gene pools in Tunisia evidenced by SSR markers and model-based Bayesian clustering
Grafting versus seed propagated apricot populations: two main gene pools in Tunisia evidenced by SSR markers and model-based Bayesian clustering
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DOI：
10.1007/s10709-010-9488-2
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发表时间：
2010-10-01
期刊：
影响因子：
1.5
通讯作者：
Audergon, Jean-Marc
中科院分区：
文献类型：
--
作者：
Bourguiba, Hedia;Khadari, Bouchaib;Audergon, Jean-Marc
杏子从中国和亚洲山区经中东和中欧引入地中海盆地。传统上存在于突尼斯，我们对了解该国杏品种的起源很感兴趣，特别是其引入的数量和地点。使用 24 个微卫星位点对一组 82 个代表性杏种质（包括 49 个嫁接品种和 33 个种子繁殖的“Bargougs”）进行基因分型，总共揭示了 135 个等位基因。使用 Structure 和 InStruct 程序以及多元方法进行的基于模型的贝叶斯聚类分析揭示了五个不同的遗传簇。簇间的遗传分化表明，簇1仅包含4个品种，与其余4个遗传簇相比，其分化程度最高，构成了所研究种质的最大部分。根据其地理起源，五个已确定的群体（北部、中部、南部、加夫萨绿洲和其他绿洲群体）在群体内具有类似的变异，分化程度较低。总体结果强调了突尼斯两个杏基因库的区别，这与品种的不同繁殖方式有关：嫁接杏和种子繁殖杏，其遗传基础很窄。我们的研究结果支持嫁接杏和种子繁殖杏具有相同起源的假设。
Apricot was introduced into the Mediterranean Basin from China and Asian mountains through the Middle-East and the Central Europe. Traditionally present in Tunisia, we were interested in accessing the origin of apricot species in the country, and in particular in the number and the location of its introductions. A set of 82 representative apricot accessions including 49 grafted cultivars and 33 seed propagated 'Bargougs' were genotyped using 24 microsatellite loci revealing a total of 135 alleles. The model-based Bayesian clustering analysis using both Structure and InStruct programs as well as the multivariate method revealed five distinct genetic clusters. The genetic differentiation among clusters showed that cluster 1, with only four cultivars, was the most differentiated from the four remaining genetic clusters, which constituted the largest part of the studied germplasm. According to their geographic origin, the five identified groups (north, centre, south, Gafsa oasis and other oases groups) enclosed a similar variation within group, with a low level of differentiation. Overall results highlighted the distinction of two apricot gene pools in Tunisia related to the different mode of propagation of the cultivars: grafted and seed propagated apricot, which enclosed a narrow genetic basis. Our findings support the assumption that grafting and seed propagated apricots shared the same origin.