Acinetobacter soli sp nov., isolated from forest soil
Acinetobacter soli sp nov., isolated from forest soil
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DOI：
10.1007/s12275-008-0118-y
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发表时间：
2008-08-01
影响因子：
3
通讯作者：
Seong, Chi Nam
中科院分区：
文献类型：
--
作者：
Kim, Duwoon;Baik, Keun Sik;Seong, Chi Nam
一种不能移动的杆状细菌，命名为菌株B1(T)，是从山上的森林土壤中分离出来的。大韩民国白云。细胞革兰氏阴性，过氧化氢酶阳性，氧化物酶阴性。主要脂肪酸为9-十八碳烯酸(C-18：1 omega 9c；42%)和十六酸(C-16：0；25.9%)和特征3(包括iso-C-15：02-OH和/或C-16：1 omega 7c；10.0%)。DNA G+C含量为44.1%。基于16SRRNA基因序列构建的系统发育树表明，菌株B1(T)与贝氏不动杆菌DSM 14961(T)亲缘关系较近(序列相似性为98.6%)，其次为鲍曼不动杆菌DSM 30007(T)(97.4%)、醋酸钙DSM 300067(97.0%)和3个基因组种(96.8%与7.6%)。表型、特性、gyrB基因序列分析和DNA-DNA亲缘关系分析将菌株B1(T)与贝氏曲霉、鲍曼曲霉和醋酸钙模式菌株区分开来。根据本研究提供的证据，菌株B1(T)是不动杆菌属的一个新种，命名为Soli不动杆菌。11月是提议的。模式菌株为BIT(=KCTC 22184(T)=JCM 15062(T))。
A non-motile and rod shaped bacterium, designated strain B1(T), seas isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C-18:1 omega 9c; 42%) and hexadecanoic acid (C-16:0; 25.9%) and summed feature 3 (comprising iso-C-15:0 2-OH and/or C-16:1 omega 7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1(T) formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961(T) (98.6% sequence similarity), followed by A. baumannii DSM 30007(T) (97.4%), A. calcoaceticus DSM 300067 (97.0%), and 3 genomic species (96.8 similar to 7.6%). Phenotypic, characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1(T) from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1(T) represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is BIT (= KCTC 22184(T)= JCM 15062(T)).