It's all relative: Centromere- versus pole-based error correction.
It's all relative: Centromere- versus pole-based error correction.
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这都是相对的：着丝粒与基于极的误差校正。
DOI：
10.1080/15384101.2015.1105701
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Maresca,ThomasJ
中科院分区：
文献类型：
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作者：
Ye,AnnaA;Maresca,ThomasJ
错误纠正是一个过程，通过这个过程，不正确的运动舞蹈微管（kt-MT）连接被破坏，这样细胞就可以在后期之前再次尝试正确的事情。把事情做好是指一种特殊的几何构型，称为双向取向，其中姐妹动粒从相反的纺锤体两极附着到动态微管（MT）上。每一条复制的染色体都必须双向定向，以最好地确保基因组的平等传递。然而，双向定位并不是固有的错误kt-MT附件形式频繁，必须解决，以避免非整倍体。典型的错误校正途径是由着丝粒富集极光B激酶（ABK）介导的，它通过磷酸化附件因子，如Ndc 80/HEC 1，降低了错误定向的动粒MT的亲和力。双向动粒施加相反的力产生张力，部分地通过抵消ABK的附着不稳定活性来稳定kt-MT附着。虽然张力对抗ABK活性的确切机制尚未完全解决，但一个称为空间定位模型的主要假设认为，动粒中的张力依赖性结构变化使附着因子进一步远离ABK，降低了它们被磷酸化的可能性，因此增加了它们对MT的亲和力。人们早就知道，误差校正经常发生在纺锤极附近，2但不清楚极地环境是否以某种方式促成了误差校正
Error correction is the process by which improper kinetochore-microtubule (kt-MT) attachments are destabilized so that the cell can take another whack at getting things right before anaphase. Getting things right refers to a specific geometric configuration called biorientation in which sister kinetochores attach to dynamic microtubules (MTs) from opposite spindle poles. Every replicated chromosome must biorient to best ensure equal transmission of the genome. However, biorientation is not inherent as erroneous kt-MT attachments form frequently and must be resolved to avoid aneuploidy.The canonical error correction pathway is mediated by the centromere-enriched aurora B kinase (ABK), which reduces the affinity of mal-oriented kinetochores for MTs by phosphorylating attachment factors such as Ndc80/HEC1. The application of opposing forces to bioriented kinetochores generates tension that stabilizes kt-MT attachments, 1 in part, by counteracting the attachment destabilizing activity of ABK. While the exact mechanism by which tension opposes ABK activity has not been entirely resolved, one major hypothesis called the spatial positioning model posits that tension-dependent structural changes in the kinetochore reposition attachment factors further from ABK, decreasing their likelihood of being phosphorylated and; therefore, increasing their affinity for MTs. It has long been known that error correction often occurs near spindle poles, 2 but it was unclear if the polar environment somehow contributed to error correction