Study of bacterial translocation from gut after paraplegia caused by spinal cord injury in rats
Study of bacterial translocation from gut after paraplegia caused by spinal cord injury in rats
复制标题
DOI：
10.1097/01.brs.0000107234.74249.cd
复制
发表时间：
2004-01-15
期刊：
影响因子：
3
通讯作者：
Li, HY
中科院分区：
文献类型：
--
作者：
Liu, JW;An, H;Li, HY
研究设计。采用正常大鼠和脊髓损伤截瘫大鼠研究肠道细菌易位。 目的。调查脊髓损伤后是否发生肠道细菌移位。背景数据摘要。已有研究表明，外伤和手术可导致胃肠麻痹；创伤后胃肠动力紊乱可能导致胃肠道细菌过度生长，增加细菌易位的发生率。然而，尚未研究脊髓损伤后细菌从肠道的移位。方法。在无菌操作下，采集血液样本进行细菌培养和内毒素测定。同时采集肠系膜淋巴结、脾脏、肝脏样本进行细菌培养。光镜和电镜观察空肠和回肠。结果。脊髓损伤并发截瘫后24小时和48小时分别出现内毒素血症和细菌移位。结论。大鼠脊髓损伤后会发生肠道细菌移位，这表明脊髓损伤截瘫患者应尽快给予抗生素，以防止潜在的细菌移位。
Study Design. Normal rats and paraplegic rats with and injured spinal cord were used to study bacterial translocation from gut.Objective. To investigate whether bacterial translocation from gut occurs after spinal cord injury.Summary of Background Data. It has been demonstrated that trauma and operation can lead to gastrointestinal paralysis; disturbance of gastrointestinal motility following trauma may cause bacterial overgrowth in gastrointestinal tract and increase the incidence of bacterial translocation. However, bacterial translocation from gut after spinal cord injury has not been studied.Methods. Under aseptic manipulation, samples of blood were collected for bacterial cultures and endotoxin determination. In the meantime, samples of mesenteric lymph node, spleen, and liver were collected for bacterial culture. The jejunum and ileum were observed by light and electron microscope.Results. Endotoxemia and bacterial translocation appeared 24 hours and 48 hours correspondingly after spinal cord injury complicated with paraplegia.Conclusion. Bacterial translocation from gut would occur after spinal cord injury in rats, which indicated that antibiotics should be administered to paraplegic patients with spinal cord injury as soon as possible to prevent potential bacterial translocation.