Lung regeneration: Implantation of fetal rat lung fragments into adult rat lung parenchyma
Lung regeneration: Implantation of fetal rat lung fragments into adult rat lung parenchyma
复制标题
DOI：
10.1016/j.jtcvs.2005.11.041
复制
发表时间：
2006-05-01
影响因子：
6
通讯作者：
Liu, MY
中科院分区：
文献类型：
--
作者：
Kenzaki, K;Sakiyama, S;Liu, MY
目的：成人肺组织在慢性破坏后再生能力有限。骨髓干细胞、视黄酸、生长因子和其他方法已尝试促进或促进这一过程。我们假设胎儿肺组织具有巨大的生长和分化潜力，可用于肺再生。方法：将Lewis大鼠肺发育假腺阶段的第17天胎儿肺组织碎片植入成年Lewis大鼠肺中。对于第1组，将胎儿肺碎片注射到成人左肺实质中；对于第2组，在部分切除左肺的情况下注射胎儿碎片；对于第3组，注射成人碎片；第4组和第5组分别将胎儿碎片植入大网膜和皮下组织。结果：植入肺实质的移植物在4周后随着肺泡腔的开放而分化，并在8周和12周后进一步进展，形态特征与新生儿肺相似。通过用印度墨水灌注确定植入物与肺循环的连接。这些变化似乎在部分肺切除的动物中进一步增强，这可能通过机械因素、可溶性因素或两者促进了植入的胎儿肺组织的成熟。胎儿肺组织在植入大网膜或皮下组织时尚未成熟。成人肺碎片在重新植入同一动物体内后并未扩张。结论：胎儿肺组织可能是进一步研究肺再生的一种选择。
Objective: The capability of regeneration of lung tissues in adults is limited after chronic destruction. Bone marrow-derived stem cells, retinoic acid, growth factors, and other approaches have been attempted to promote or facilitate this process. We hypothesized that fetal lung tissues, with great potential for growth and differentiation, could be used for lung regeneration.Methods: Day 17 fetal lung tissue fragments at the pseudoglandular stage of lung development from Lewis rats were implanted into adult Lewis rat lungs. For group 1, fetal lung fragments were injected into the adult left lung parenchyma; for group 2, fetal fragments were injected with the left lung partially resected; for group 3, adult fragments were injected; and for groups 4 and 5, fetal fragments were implanted into the omentum and subcutaneous tissue, respectively.Results: The grafts implanted into pulmonary parenchyma were differentiated with opening of the alveolar space after 4 weeks and were advanced further with morphologic features similar to those of neonatal lungs after 8 and 12 weeks. The implants were connected with pulmonary circulation determined by means of perfusion with India ink. These changes appeared to be further enhanced in animals with partial lung resection that might have facilitated the maturation of implanted fetal lung tissues through mechanical factors, soluble factors, or both. Fetal lung tissues did not mature when implanted into the omentum or subcutaneous tissue. Adult lung fragments did not expand after being reimplanted back into the same animal.Conclusions: Fetal lung tissue might be an option for further investigation into lung regeneration.