Sex-specific patterns of galanin, cholecystokinin, and substance P expression in neurons of the principal bed nucleus of the stria terminalis are differentially reflected within three efferent preoptic pathways in the juvenile rat.
Sex-specific patterns of galanin, cholecystokinin, and substance P expression in neurons of the principal bed nucleus of the stria terminalis are differentially reflected within three efferent preoptic pathways in the juvenile rat.
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幼年大鼠终纹主床核神经元中甘丙肽、胆囊收缩素和 P 物质表达的性别特异性模式在幼年大鼠的三个传出视前通路中有所不同。
DOI：
10.1002/cne.10841
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Simerly,RichardB
中科院分区：
文献类型：
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作者：
Polston,EvaK;Simerly,RichardB
终纹主床核 (BSTp) 中的神经元整合与生殖相关的激素和感觉信息，并将这些信息传输到调节神经内分泌和行为功能的下丘脑核团。神经肽甘丙肽 (GAL)、胆囊收缩素 (CCK) 和 P 物质 (SP) 在 BSTp 神经元中高度表达，并受到性类固醇的差异调节。目前的实验研究了发育或青春期前后激素介导的 GAL、CCK 和 SP 表达变化是否反映在调节促性腺激素分泌和性行为的视前结构的传出通路中。对雄性和雌性幼年大鼠 BSTp 的投影进行顺行标记，并结合前腹侧室周前核 (AVPV) 和内侧视前核的中央和内侧部分（分别为 MPNc、MPNm）中含有 GAL、CCK 和 SP 的纤维的免疫组织化学标记，揭示了每个区域独特的性别差异。在 AVPV 中，雄性菜豆白细胞凝集素标记的纤维密度高于雌性，并且雄性这些纤维中含有 GAL 的比例高于雌性。相比之下，女性中从 BSTp 投射到 MPNc 的纤维比男性更可能含有 SP。用外源性睾酮和雌二醇治疗已切除性腺的青春期前后男性和女性，并没有改变所检查的三个脑区中任何一个区域中含有 GAL、CCK 或 SP 的纤维的密度。总的来说，这些结果表明 BSTp 投射中的神经肽表达模式是在发育过程中建立的，从而为每个投射途径产生独特、稳定和性别特异性的化学结构特征。 J.Comp。内罗尔。 465:551–559，2003 年。© 2003 Wiley-Liss, Inc.
Neurons in the principal bed nucleus of the stria terminalis (BSTp) integrate hormonal and sensory information associated with reproduction and transmit this information to hypothalamic nuclei that regulate neuroendocrine and behavioral functions. The neuropeptides galanin (GAL), cholecystokinin (CCK), and substance P (SP) are highly expressed in BSTp neurons and are differentially regulated by sex steroids. The current experiments investigated whether developmental or peripubertal hormone‐mediated changes in GAL, CCK, and SP expression are reflected within efferent pathways to the preoptic structures that regulate gonadotropin secretion and sexual behavior. Anterograde labeling of projections from the BSTp of male and female juvenile rats combined with immunohistochemical labeling of GAL‐, CCK‐, and SP‐containing fibers in the anteroventral periventricular preoptic nucleus (AVPV) and the central and medial divisions of the medial preoptic nucleus (MPNc, MPNm, respectively) revealed unique sex differences in each region. In the AVPV,Phaseolus vulgarisleucoagglutinin‐labeled fibers were seen at a greater density in males than in females, and higher percentages of these fibers contained GAL in males than in females. In contrast, fibers projecting from the BSTp to the MPNc were more likely to contain SP in females than in males. Treatment of gonadectomized, peripubertal males and females with exogenous testosterone and estradiol did not alter the densities of GAL‐, CCK‐, or SP‐containing fibers in any of the three brain areas examined. Collectively, these results suggest that patterns of neuropeptide expression in BSTp projections are established during development, resulting in a distinct, stable, and sex‐specific chemoarchitectural profile for each projection pathway. J. Comp. Neurol. 465:551–559, 2003. © 2003 Wiley‐Liss, Inc.