Sodium sulfite is a potential hypoxia inducer that mimics hypoxic stress in Caenorhabditis elegans
Sodium sulfite is a potential hypoxia inducer that mimics hypoxic stress in Caenorhabditis elegans
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亚硫酸钠是一种潜在的缺氧诱导剂，可模拟秀丽隐杆线虫的缺氧应激
DOI：
10.1007/s00775-010-0723-1
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发表时间：
2011-02-01
影响因子：
3
通讯作者：
Zhang, Chenggang
中科院分区：
文献类型：
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作者：
Jiang, Bin;Ren, Changhong;Zhang, Chenggang
物理和化学缺氧已广泛应用于缺氧损伤的研究；然而，这两种缺氧模型都有其自身的局限性。生理缺氧通常难以控制和维持。化学缺氧通常由化学模拟缺氧剂（如CoCl(2)）引起，可能导致重金属中毒或构成安全威胁。为了建立一个更合适的缺氧模型，我们重点研究了亚硫酸钠(Na(2)SO(3))，并评估了其去除水溶液中溶解氧的能力。我们的结果表明，亚硫酸钠成功地诱导了缺氧条件。可溶亚硫酸钠的浓度可以很容易地控制亚硫酸钠溶液的缺氧程度和保质期。此外，我们使用亚硫酸钠在秀丽隐杆线虫中建立了缺氧模型。与物理缺氧类似，亚硫酸钠溶液诱导线虫缺氧相关死亡，导致细胞形态缺陷和线虫缺氧诱导因子1稳定。综上所述，我们的数据表明，亚硫酸钠是一种潜在的缺氧诱导剂，可以模拟秀丽隐杆线虫的缺氧应激。
Physical and chemical hypoxia have been widely used in the study of hypoxic injury; however, both of these hypoxia models have their own limitations. Physical hypoxia is usually difficult to control and maintain. Chemical hypoxia, which is usually induced by chemical hypoxia-mimicking agents, such as CoCl(2), may result in heavy metal toxicity or impose security threats. To develop a more suitable hypoxia model, we focused on sodium sulfite (Na(2)SO(3)) and evaluated its ability to remove dissolved oxygen in aqueous solutions. Our results showed that sodium sulfite successfully induced hypoxic conditions. The degree of hypoxia and the guarantee period of the sodium sulfite solution could be easily controlled by the concentration of soluble sodium sulfite. In addition, we used sodium sulfite to create a hypoxia model in Caenorhabditis elegans. Similar to physical hypoxia, the sodium sulfite solutions induced hypoxia-related death in the worms and led to morphologic cell defects and C. elegans hypoxia inducible factor 1 stabilization. Taken together, our data show that sodium sulfite is a potential hypoxia inducer that mimics hypoxic stress in C. elegans.