Decreased undercarboxylated osteocalcin in children with type 2 diabetes mellitus
Decreased undercarboxylated osteocalcin in children with type 2 diabetes mellitus
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DOI：
10.1515/jpem-2015-0417
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发表时间：
2016-08
影响因子：
1.4
通讯作者：
J. Takaya;Y. Tanabe;Y. Kuroyanagi;K. Kaneko
中科院分区：
文献类型：
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作者：
J. Takaya;Y. Tanabe;Y. Kuroyanagi;K. Kaneko
摘要背景：骨钙素（OC）是一种由成骨细胞分泌的骨特异性蛋白，常被用作骨形成的生物标志物。OC经历翻译后羧化以产生羧化骨钙蛋白（Gla-OC）和欠羧化骨钙蛋白（uc-OC）分子。本研究的目的是通过评估肥胖和2型糖尿病（DM 2）儿童的OC、离子阳离子和糖代谢标志物来探讨骨与糖代谢之间的关系。研究方法：受试者包括9名DM 2儿童[6名男性，3名女性;年龄15.7±4.1岁;病程3.2±1.2年]、18名单纯性肥胖儿童[12名男性，6名女性;年龄12.6±4.1岁]和12名对照[8名男性，4名女性;年龄12.3±3.2岁]。采用酶联免疫吸附试验（ELISA）测定血清Gla-OC和uc-OC水平。结果：DM 2患者（0.65±0.46 ng/mL）的uc-OC水平（1.11±0.55 ng/mL）低于对照组（1.25±0.49 ng/mL），而非肥胖患者（1.11±0.55 ng/mL）。所有受试者的血清uc-OC与平均血糖水平（r=-0.447，p=0.013）和血红蛋白A1 c（HbA 1c）（r=-0.455，p=0.012）呈负相关。血清Gla-OC与血清碱性磷酸酶（r=0.601，p<0.001）和无机磷（r=0.686，p<0.001）相关，与年龄呈负相关（r=-0.383，p=0.030）。DM 2受试者的平均血清离子镁低于对照组。平均血清离子钙在肥胖者高于对照组。在所有受试者中，平均血清离子镁与平均血清葡萄糖水平呈负相关。结论：成骨细胞衍生蛋白OC，尤其是uc-OC，可能通过与血糖稳态相关而在糖尿病的病理生理学中发挥作用。
Abstract Background: Osteocalcin (OC) is a bone-specific protein secreted by osteoblasts and often used as a bone formation biomarker. OC undergoes post-translational carboxylation to yield carboxylated osteocalcin (Gla-OC) and undercarboxylated osteocalcin (uc-OC) molecules. The aim of this study was to explore the association between bone and glucose metabolism by evaluating OC, ionized cations, and markers of glucose metabolism in children with obesity and type 2 diabetes mellitus (DM2). Methods: The subjects were nine children with DM2 [six males, three females; age 15.7±4.1 years; duration of disease 3.2±1.2 years], 18 children with simple obesity [12 males, six females; age 12.6±4.1 years], and 12 controls [eight males, four females; age 12.3±3.2 years]. Serum Gla-OC and uc-OC levels were determined using an enzyme-linked immunosorbent assay (ELISA). Results: Patients with DM2 (0.65±0.46 ng/mL), but not with obesity (1.11±0.55 ng/mL), had lower uc-OC levels than controls (1.25±0.49 ng/mL). Serum uc-OC was negatively correlated with mean serum glucose levels (r=–0.447, p=0.013) and hemoglobin A1c (HbA1c) (r=–0.455, p=0.012) in all subjects. Serum Gla-OC was correlated with serum alkaline phosphatase (r=0.601, p<0.001) and inorganic phosphorus (r=0.686, p<0.001), yet negatively correlated with age (r=–0.383, p=0.030). Mean serum ionized magnesium was lower in DM2 subjects than in controls. Mean serum ionized calcium was higher in obese subjects than in controls. In all subjects, mean serum ionized magnesium was negatively correlated with mean serum glucose levels. Conclusions: Osteoblast-derived protein OC, especially uc-OC, may have a role in the pathophysiology of diabetes by being associated with blood glucose homeostasis.