Spatially resolved biologic information from in vivo EPRI, OMRI, and MRI.
Spatially resolved biologic information from in vivo EPRI, OMRI, and MRI.
复制标题
来自体内 EPRI、OMRI 和 MRI 的空间分辨生物信息。
DOI：
10.1089/ars.2007.1638
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发表时间：
2007
影响因子：
6.6
通讯作者：
Krishna,MuraliC
中科院分区：
文献类型：
--
作者：
Matsumoto,Ken-ichiro;Subramanian,Sankaran;Murugesan,Ramachandran;Mitchell,JamesB;Krishna,MuraliC
EPR光谱可以基于EPR光谱特性的变化（即，强度，线宽，超精细分裂，和自由基探针的光谱形状。EPR成像（EPRI）可以使用磁场梯度的几种组合来获得这样的谱分量的1D-3D空间分布。Overhauser增强MRI（OMRI）是一种电子和核自旋的双共振技术。由于Overhauser增强依赖于电子自旋的横向弛豫速率，因此OMRI可以沿着高分辨率MR图像间接提供pO 2信息。MRI还可以间接检测自由基造影剂的顺磁行为。涉及顺磁物质（即，EPRI、OMRI和MRI）具有获得最大5D信息（即，理论上，3D空间+ 1D光谱+ 1D时间维度）。为了获得合适的维数，必须考虑几个因素，例如EPR光谱信息、空间分辨率、时间分辨率。在这篇综述中，EPRI，OMRI和MRI的研究生物系统的应用进行了评估，研究人员应用的方法选择和测量模式，以特定的实验系统。
EPR spectroscopy can give biologically important information, such as tissue redox status, pO2, pH, and microviscosity, based on variation of EPR spectral characteristics (i.e., intensity, linewidth, hyperfine splitting, and spectral shape of free radical probes. EPR imaging (EPRI) can obtain 1D–3D spatial distribution of such spectral components using several combinations of magnetic field gradients. Overhauser enhanced MRI (OMRI) is a double-resonance technique of electron and nuclear spins. Because the Overhauser enhancement depends on transverse relaxation rate of the electron spin, OMRI can provide pO2information indirectly, along with a high-resolution MR image. MRI can also indirectly detect paramagnetic behaviors of free radical contrast agents. Imaging techniques and applications relating to paramagnetic species (i.e., EPRI, OMRI, and MRI) have the potential to obtain maximally 5D information (i.e., 3D spatial + 1D spectral + 1D temporal dimensions, theoretically). To obtain suitable dimensionality, several factors, such as the EPR spectral information, spatial resolution, temporal resolution, will have to be taken into account. For this review, the EPRI, OMRI, and MRI applications for the study biological systems were evaluated for researchers to apply the method of choice and the mode of measurements to specific experimental systems.