Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes
Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes
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DOI：
10.1002/1097-0061(20010315)18:4
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发表时间：
2001-03
期刊：
影响因子：
2.6
通讯作者：
K. Lecoq;I. Belloc;C. Des̀granges;Bertrand Daignan-Fornier
中科院分区：
文献类型：
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作者：
K. Lecoq;I. Belloc;C. Des̀granges;Bertrand Daignan-Fornier
对酿酒酵母基因组测序发现了一个开放阅读框（YJR 105 w），编码一种与其他物种腺苷激酶高度相似的蛋白质。该基因（重命名为ADO 1）的破坏影响了S-腺苷甲硫氨酸（S-adenosyl methionine，S-Met）作为嘌呤来源的利用，并导致粗提物中腺苷激酶活性的严重降低。此外，敲除ADO 1导致培养基中的腺苷排泄和对毒性腺苷类似物虫草素的抗性。从这些数据中，我们得出结论，ADO 1编码酵母腺苷激酶。我们还表明，ADO 1并没有发挥主要作用，在腺嘌呤利用酵母，我们建议，腺苷激酶的生理作用，在S。酿酒酵母的主要用途是回收甲基循环产生的腺苷。版权所有© 2001约翰威利父子有限公司。
Sequencing of the Saccharomyces cerevisiae genome revealed an open reading frame (YJR105w) encoding a putative protein highly similar to adenosine kinases from other species. Disruption of this gene (renamed ADO1) affected utilization of S‐adenosyl methionine (AdoMet) as a purine source and resulted in a severe reduction of adenosine kinase activity in crude extracts. Furthermore, knock‐out of ADO1 led to adenosine excretion in the medium and resistance to the toxic adenosine analogue cordycepin. From these data we conclude that ADO1 encodes yeast adenosine kinase. We also show that ADO1 does not play a major role in adenine utilization in yeast and we propose that the physiological role of adenosine kinase in S. cerevisiae could primarily be to recycle adenosine produced by the methyl cycle. Copyright © 2001 John Wiley & Sons, Ltd.