Expression of BMI-1 in normal skin and inflammatory and neoplastic skin lesions
Expression of BMI-1 in normal skin and inflammatory and neoplastic skin lesions
复制标题
DOI：
10.1111/j.1600-0560.2006.00587.x
复制
发表时间：
2007-02-01
影响因子：
1.7
通讯作者：
Pammer, Johannes
中科院分区：
文献类型：
--
作者：
Reinisch, Christina M.;Uthman, Aumaid;Pammer, Johannes
背景资料：BMI-1参与干细胞的维持，并作为癌基因在淋巴瘤和实体癌中发挥作用，通过下调p16 ink 4a发挥作用。我们研究了BMI-1在正常和炎症皮肤以及基底细胞癌（BCC）和鳞状细胞癌（SCC）中的表达谱。方法：采用免疫组织化学和免疫荧光法测定BMI-1的表达，并进行半定量评价。BMI-1在基底和有时基底上角质形成细胞的细胞核中弱表达，在皮脂腺的基底细胞中，在隆突区和毛囊外根鞘中弱至中度，在汗腺中强烈。BCCs表现出强烈和弥漫性的BMI-1表达，而SCC则不均匀地表达BMI-1。结论：BMI-1的表达标志着毛囊内的干细胞。由于BMI-1也存在于基底上角质形成细胞和各种特化细胞中，BMI-1的分布仅部分反映了干细胞区室的已知分布。BMI-1在BCC中强烈过表达，BCC是与音刺猬通路失调有关的肿瘤，已被证明可以上调BMI-1，这表明BMI-1癌基因在其发病机制中发挥了作用。
Background: BMI-1 is involved in the maintenance of stem cells and functions as an oncogene in both lymphomas and solid carcinomas, acting by downregulation of p16ink4a. We have investigated the expression profile of BMI-1 in normal and inflamed skin as well as in basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs).Methods: BMI-1 expression was determined by immunohistochemistry and immunofluorescence, and evaluated semiquantitatively.Results: BMI-1 was weakly expressed in nuclei of basal and sometimes suprabasal keratinocytes, in basal cells of sebaceous glands, weakly to moderately in the bulge area and the external root sheath of hair follicles, and strongly in sweat glands. Whereas BCCs showed strong and diffuse BMI-1 expression, SCCs expressed BMI-1 heterogeneously. Strong cytoplasmic expression of BMI-1 was found in dividing cells.Conclusions: BMI-1 expression marks stem cells within the hair follicle. As BMI-1 was also found in suprabasal keratinocytes and a variety of specialized cells, the distribution of BMI-1 only partly reflects the known distribution of stem cell compartments. BMI-1 is strongly overexpressed in BCCs, tumors linked to dysregulation of the sonic hedgehog pathway, which has been shown to upregulate BMI-1, suggesting a contribution of the BMI-1 oncogene in their pathogenesis.