The ethanol extract of Involcucnirn castaneae ameliorated ovalbumin-induced airway inflammation and smooth muscle thickening in guinea pigs
The ethanol extract of Involcucnirn castaneae ameliorated ovalbumin-induced airway inflammation and smooth muscle thickening in guinea pigs
复制标题
板栗乙醇提取物可改善豚鼠卵清蛋白诱导的气道炎症和平滑肌增厚
DOI：
10.1016/j.jep.2018.10.027
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发表时间：
2019
影响因子：
5.4
通讯作者：
Wang Jianping
中科院分区：
文献类型：
--
作者：
Ye Yujie;Mo Shuyuan;Feng Wenya;Ye Xiaochun;Shu Xiang;Long Yuxi;Guan Yeli;Huang Jiangeng;Wang Jianping
民族药理学相关性栗叶总黄酮（Involucrum castaneae，IC）在中国民间用于治疗各种肺部疾病，以及其化痰和抗炎的特性。本实验的目的是验证IC对卵清蛋白（ovalbumin，OVA）诱导的哮喘豚鼠气道炎症、反应性的影响。材料与方法研究了板栗叶乙醇提取物（EEIC）对卵白蛋白（OVA）诱导的豚鼠过敏性气道反应的保护作用。诱发豚鼠过敏性哮喘后，记录哮喘潜伏期。采用酶联免疫吸附法（ELISA）检测哮喘过敏原中免疫球蛋白E（IgE）、白细胞介素5（IL-5）、神经生长因子（NGF）和γ干扰素（IFN-γ）的水平。逆转录聚合酶链反应（RT-PCR）检测哮喘豚鼠肺组织中IL-5 mRNA的表达。肺组织石蜡切片分析病理变化。结果EEIC能降低哮喘豚鼠支气管肺泡灌洗液（BALF）和外周血（PB）中嗜酸性粒细胞（EOS）的数量。同时显著降低炎症相关因子IgE和IL-5水平，降低肺组织IL-5 mRNA表达，升高IFN-γ水平。肺组织石蜡切片病理检查显示EEIC可减轻支气管平滑肌增厚，减轻各种炎性细胞对组织的浸润损伤。EEIC中的黄酮类、萜类和酚类化合物的存在可能是导致这些activity.ConclusionIC减轻豚鼠OVA致敏过敏性哮喘的气道炎症和平滑肌增厚的原因。阐述了中药IC的传统功效和物质基础，为进一步开发奠定了基础。
Ethnopharmacological relevanceInvolucrum castaneae(IC)is used in Chinese folk medicine to treat various lung diseases, as well as for its reducing phlegm and anti-inflammatory properties.Aim of the studyThe purpose of this experiment is to verify the effect of IC on airway inflammation, responsiveness in ovalbumin (OVA)-induced asthmatic guinea pigs. The main chemical components of IC were also analyzed.Materials and methodsThe potential of the ethanol extract ofInvolucrum castaneae(EEIC) to protect against OVA-induced allergic airway response in guinea pigs was investigated. The latency of asthma in guinea pigs were recorded after the allergic asthma induced. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of immunoglobulin E (IgE), interleukin-5 (IL-5), nerve growth factor (NGF) and interferon-γ (IFN-γ) in asthma allergy. Reverse transcription-PCR (RT-PCR) was used to detect the expression of IL-5 mRNA in asthmatic guinea pig lungs. Paraffin sections of lung tissue were used to analyze pathological changes. The total flavonoid content was determined and the chemical components were analyzed by LC-MS/MS.ResultsIt was found that EEIC was able to reduce the number of eosinophil (EOS) in bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) in the guinea pig model of OVA -induced asthma. Meanwhile, it also significantly reduced the levels of inflammation-related factors IgE and IL-5, decreased the expression of IL-5 mRNA in lung tissue, and increased the level of IFN-γ. Pathological examination of paraffin section of lung tissue showed that EEIC can reduce the thickening of bronchial smooth muscle and reduce the infiltration damage of tissues by various inflammatory cells. The presence of flavonoids, terpenoids and phenolic compounds in EEIC might be responsible for these activities.ConclusionIC alleviated airway inflammation and smooth muscle thickening in guinea pigs with OVA-sensitized allergic asthma. The paper explains the traditional efficacy and material basis of IC and lays a foundation for further development.