HELPER T-CELLS INFILTRATING HUMAN RENAL-CELL CARCINOMAS HAVE THE PHENOTYPE OF ACTIVATED MEMORY-LIKE T-LYMPHOCYTES
HELPER T-CELLS INFILTRATING HUMAN RENAL-CELL CARCINOMAS HAVE THE PHENOTYPE OF ACTIVATED MEMORY-LIKE T-LYMPHOCYTES
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DOI：
10.1097/00002371-199501000-00005
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发表时间：
1995-01-01
影响因子：
3.9
通讯作者：
ROSENBERG, SA
中科院分区：
文献类型：
--
作者：
ALEXANDER, RB;FITZGERALD, EB;ROSENBERG, SA
人肾细胞癌的特征是含有许多 T 淋巴细胞的炎症浸润。通过在白细胞介素（IL）-2中培养来从此类肿瘤中培养T细胞的尝试已经产生了异质细胞群，其具有通过类似的外周血单核细胞培养物获得的淋巴因子激活杀伤细胞的典型功能特征。我们检查了一组 T 淋巴细胞上表达的表面标记，以确定浸润人肾细胞癌的 CD4(+) T 细胞是否与外周血单核细胞中的不同。通过流式细胞术分析，一组新鲜消化的人肾细胞癌原发性和转移性肿瘤中的CD4（+）T细胞表达激活标记物CD69和HLA-DR，并且与外周血CD4（+）T细胞相比，CD45RO表达增加，CD45RA表达相应减少。这表明浸润肾细胞癌的CD4(+) T细胞被激活并遇到了抗原。然而，IL-2Rα链（CD25）的表达在肿瘤浸润CD4（+）T细胞和外周血CD4（+）T细胞中没有差异，这表明浸润人肾细胞癌的T细胞的增殖能力可能受到阻碍。培养的肾细胞癌肿瘤浸润淋巴细胞 (TIL) 通常无法证明肿瘤特异性反应性，可能是由于此类细胞无法在 IL-2 中生长。
Human renal cell carcinomas are characterized by an inflammatory infiltrate containing many T lymphocytes. Attempts to grow T cells from such tumors by culture in interleukin (IL)-2 have yielded heterogeneous populations of cells with functional characteristics typical of lymphokine-activated killer cells obtained by similar culture of cells from peripheral blood mononuclear cells. We examined a panel of surface markers expressed on T lymphocytes to determine if the CD4(+) T cells infiltrating human renal cell carcinomas are different from those in peripheral blood mononuclear cells. By flow cytometry analysis the CD4(+) T cells in a panel of freshly digested human renal cell carcinoma primary and metastatic tumors expressed the activation markers CD69 and HLA-DR and manifested an increase in CD45RO and a reciprocal decrease in CD45RA expression as compared with peripheral blood CD4(+) T cells. This suggests that CD4(+) T cells infiltrating renal cell carcinomas are activated and have encountered antigen. However, the expression of the IL-2R a chain (CD25) was not different in tumor-infiltrating CD4(+) T cells and peripheral blood CD4(+) T cells, suggesting that T cells infiltrating human renal cell carcinomas may have a block in proliferative capacity. The general failure of cultured tumor-infiltrating lymphocyte (TIL) from renal cell carcinoma to demonstrate tumor-specific reactivity may be due to the failure of such cells to grow in IL-2.