Activation of the SCPx promoter in mouse adrenocortical Y1 cells
Activation of the SCPx promoter in mouse adrenocortical Y1 cells
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DOI：
10.1016/j.bbrc.2007.03.194
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发表时间：
2007-06-01
影响因子：
3.1
通讯作者：
McLean, Mark P.
中科院分区：
文献类型：
--
作者：
Lopez, Dayami;Niesen, Melissa;McLean, Mark P.
固醇载体蛋白X(SCPx)是一种具有脂质转移和硫解酶活性的过氧体蛋白。用cAMP处理小鼠肾上腺Y1细胞24小时，可显著诱导SCPx基因的表达。报告基因研究表明，cAMP和SF-1处理能够激活SCPx启动子。序列分析显示存在三个类固醇生成因子-1(SF-1)结合基序(命名为SFB1、SFB2和SFB3)和一个Cre。在凝胶迁移率改变实验中，只有SFB1和SFB3能与重组SF-1蛋白结合。在Y1核抽提物存在下，Cre能形成DNA/蛋白质复合体。突变分析研究表明，通过cAMP处理，SCPx启动子的完全激活需要SFB3。SF-1和cAMP对SCPx基因的调控机制与观察到的其他类固醇合成基因的调控机制相似。(C)2007 Elsevier Inc.保留所有权利。
Sterol carrier protein X (SCPx) is a peroxisomal protein with both lipid transfer and thiolase activity. Treatment of mouse adrenal Y1 cells with cAMP for 24 h caused a significant induction of SCPx mRNA levels. Reporter gene studies demonstrated that treatment with cAMP and SF-1 was able to activate the SCPx promoter. Sequence analysis revealed the presence of three putative steroidogenic factor-1 (SF-1) binding motifs (designated SFB1, SFB2, and SFB3) and one CRE. Only SFB1 and SFB3 were able to bind recombinant SF-1 protein in electrophoretic mobility shift assays. The CRE was able to form a DNA/protein complex in the presence of Y1 nuclear extracts. Mutational analysis studies demonstrated that SFB3 is required for full activation of the SCPx promoter by cAMP treatment. Regulation of the SCPx gene by SF-1 and cAMP is similar to the regulatory mechanisms observed for other steroidogenic genes. (c) 2007 Elsevier Inc. All rights reserved.