Excitatory response of prefrontal cortical fast-spiking interneurons to ventral tegmental area stimulation in vivo.

Excitatory response of prefrontal cortical fast-spiking interneurons to ventral tegmental area stimulation in vivo.
复制标题

前额皮质快速尖峰中间神经元对体内腹侧被盖区刺激的兴奋反应。

DOI：
10.1002/syn.20255
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发表时间：
2006
期刊：
Synapse (New York, N.Y.)
影响因子：
--
通讯作者：
O'Donnell,Patricio
O'Donnell,Patricio
中科院分区：
--
文献类型：
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作者：
Tseng,KueiY;Mallet,Nicolas;Toreson,KathyL;LeMoine,Catherine;Gonon,François;O'Donnell,Patricio

文献摘要

相似文献

前额皮质（PFC）锥体神经元（PN）和快速尖峰中间神经元（FSI）接受来自腹侧被盖区（VTA）的多巴胺能（DA）和非DA输入。虽然PN对VTA刺激和DA的反应已经被广泛研究，但FSI对中脑皮层激活的反应却知之甚少。我们利用化学VTA刺激下内侧PFC PN和FSI的单次和双次体内近细胞记录来探讨这个问题。电生理特征结合神经生物素染色和小白蛋白免疫组织化学可以将记录的细胞鉴定为FSI或PN。在所有测试的FSI （n= 7）中，向VTA注射NMDA增加了放电，而大多数PN（7/11）有抑制反应。此外，通过FSI - PN配对记录观察到FSI激发与PN抑制的时间过程相匹配（n= 5）。这些对中皮层激活的不同电生理反应可能反映了PFC gaba能中间神经元参与沉默PN。因此，中皮层系统可以通过同时影响FSI和PN放电来提供PFC电路的关键控制。突触59:12 - 417,2006。©2006 Wiley‐Liss, Inc。
Prefrontal cortical (PFC) pyramidal neurons (PN) and fast spiking interneurons (FSI) receive dopaminergic (DA) and non‐DA inputs from the ventral tegmental area (VTA). Although the responses of PN to VTA stimulation and DA administration have been extensively studied, little is known about the response of FSI to mesocortical activation. We explored this issue using single and double in vivo juxtacellular recordings of medial PFC PN and FSI with chemical VTA stimulation. Electrophysiological characteristics combined with Neurobiotin staining and parvalbumin immunohistochemistry allowed identification of recorded cells as FSI or PN. NMDA injection into the VTA increased firing in all FSI tested (n= 7), whereas most PN (7/11) responded with an inhibition. Furthermore, FSI excitation matching the temporal course of PN inhibition was observed with FSI–PN paired recordings (n= 5). These divergent electrophysiological responses to mesocortical activation could reflect PFC GABAergic interneurons contributing to silencing PN. Thus, the mesocortical system could provide a critical control of PFC circuits by simultaneously affecting FSI and PN firing. Synapse 59:412–417, 2006. © 2006 Wiley‐Liss, Inc.