Genetic mapping of agronomic traits in false flax (Camelina sativa subsp sativa)
Genetic mapping of agronomic traits in false flax (Camelina sativa subsp sativa)
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DOI：
10.1139/g06-117
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发表时间：
2006-12-01
期刊：
影响因子：
3.1
通讯作者：
Snowdon, R. J.
中科院分区：
文献类型：
--
作者：
Gehringer, A.;Friedt, W.;Snowdon, R. J.
十字花科油料植物亚麻(Camelirea sativa subsp.)Sativa)具有许多有价值的农艺属性，使其成为一种适合紧密轮作的春播替代作物。亚麻油富含多不饱和C18-脂肪酸，是油脂化学工业宝贵的可再生原料。由于在整个20世纪对这种作物的兴趣微乎其微，育种努力一直有限。本研究利用扩增片段长度多态(AFLP)分子标记，以2个表型不同的亲本品种‘林多’和‘利卡拉’杂交的重组自交系为材料，利用AFLP标记构建了水稻遗传图谱。3个甘蓝型油菜简单重复序列(SSR)标记也被整合到该图谱中，其中1个标记与甘蓝型油菜和甘蓝型油菜的含油量基因座连锁。另外55个SSR引物组合显示出单态扩增产物，表明与十字花科植物的部分基因组同源性。利用3年来不同施肥处理(0和80 kg N/ha)在多个地点的田间试验数据，利用该图谱对籽粒产量、含油量、千粒重和株高进行了QTL定位。一些产量QTL只有在不处理时才被检测到，这些QTL可能代表了亚麻在低养分土壤上的竞争能力。这些结果代表了未来分子标记辅助育种的起点。
The crucifer oilseed plant false flax (Camelirea sativa subsp. sativa) possesses numerous valuable agronomic attributes that make it attractive as an alternative spring-sown crop for tight crop rotations. The oil of false flax is particularly rich in polyunsaturated C18-fatty acids, making it a valuable renewable feedstock for the oleochemical industry. Because of the minimal interest in the crop throughout the 20th century, breeding efforts have been limited. In this study, a genetic map for C. sativa was constructed, using amplified fragment length polymorphism (AFLP) markers, in a population of recombinant inbred lines that were developed, through single-seed descent, from a cross between 'Lindo' and 'Licalla', 2 phenotypically distinct parental varieties. Three Brassica simple sequence repeat (SSR) markers were also integrated into the map, and 1 of these shows linkage to oil-content loci in both C. sativa and Brassica napus. Fifty-five other SSR primer combinations showed monomorphic amplification products, indicating partial genome homoeology with the Brassica species. Using data from field trials with different fertilization treatments (0 and 80 kg N/ha) at multiple locations over 3 years, the map was used to localize quantitative trait loci (QTLs) for seed yield, oil content, 1000-seed mass, and plant height. Some yield QTLs were found only with the NO treatment, and might represent loci contributing to the competitiveness of false flax in low-nutrient soils. The results represent a starting point for future marker-assisted breeding.