Gastrin and somatostatin secretion by perfused rat stomach: functional linkage of antral peptides.
Gastrin and somatostatin secretion by perfused rat stomach: functional linkage of antral peptides.
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灌注大鼠胃分泌胃泌素和生长抑素：胃窦肽的功能连接。
DOI：
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发表时间：
1980
影响因子：
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通讯作者：
G. Makhlouf
中科院分区：
文献类型：
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作者：
B. Saffouri;G. Weir;K. Bitar;G. Makhlouf
采用离体大鼠胃血管灌流，研究胃泌素和生长抑素分泌的极性、动力学和化学计量以及两种胃窦肽的相互作用。胃泌素（79%）和生长抑素（95%）的分泌主要在循环中。乙酰甲胆碱（5 × 10（-8）至5 × 10（-4）M）可使胃泌素分泌呈剂量依赖性双相增加。最大胃泌素反应（高于基础水平434 ± 89%; P <0.001）被10（-8）M阿托品部分抑制，被10（-7）M阿托品完全抑制。乙酰甲胆碱对生长抑素分泌产生剂量依赖性抑制，这种抑制作用可被阿托品阻断。在基础状态和乙酰甲胆碱或前列腺素E2输注期间，胃泌素和生长抑素的分泌之间存在反比关系;后者对胃泌素和生长抑素的分泌产生与乙酰甲胆碱相反的影响。这些数据，连同其他地方报道的数据，即生长抑素抗血清刺激灌流胃中的胃泌素分泌，与胃生长抑素分泌对基础胃泌素分泌产生持续抑制的假设一致，并且胃泌素分泌的刺激可能部分由生长抑素分泌的抑制介导。
The isolated vascularly perfused rat stomach was used to study the polarity, kinetics, and stoichiometry of gastrin and somatostatin secretion and the interaction of the two antral peptides. The secretion of gastrin (79%) and somatostatin (95%) was predominantly in the circulation. Methacholine (5 x 10(-8) to 5 x 10(-4) M) produced a biphasic dose-dependent increase in gastrin secretion. The maximal gastrin response (434 +/- 89% above basal levels; P less than 0.001) was partially inhibited by 10(-8) M atropine and completely inhibited by 10(-7) M atropine. Methacholine produced a dose-dependent inhibition of somatostatin secretion; the inhibition was blocked by atropine. An inverse relationship between the secretion of gastrin and somatostatin was noted in the basal state and during infusion of methacholine or prostaglandin E2; the latter had effects on gastrin and somatostatin secretion opposite to those of methacholine. The data, together with data reported elsewhere that somatostatin antiserum stimulates gastrin secretion in the perfused stomach, are consistent with the hypothesis that gastric somatostatin secretion exerts a continuous restraint on basal gastrin secretion and that stimulation of gastrin secretion may be mediated in part by inhibition of somatostatin secretion.