Direct interaction between the catalytic subunit of the calmodulin-sensitive adenylate cyclase from bovine brain with 125I-labeled wheat germ agglutinin and 125I-labeled calmodulin.
Direct interaction between the catalytic subunit of the calmodulin-sensitive adenylate cyclase from bovine brain with 125I-labeled wheat germ agglutinin and 125I-labeled calmodulin.
复制标题
牛脑钙调蛋白敏感腺苷酸环化酶的催化亚基与 125I 标记的麦芽凝集素和 125I 标记的钙调蛋白之间的直接相互作用。
DOI：
10.1021/bi00388a038
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Storm,DR
中科院分区：
文献类型：
--
作者：
Minocherhomjee,M;Selfe,S;Flowers,NJ;Storm,DR
Materials and MethodsGppNHp, wheat germ agglutinin-agarose, and adenosine were obtained from PL Biochemicals. ATP, cAMP, and protein standards were from Sigma.[a-32P]-and [3H] cAMP were purchased from New England Nuclear and International Chemical Nuclear, respectively. All other reagents were of the purest available grade from commercial sources. Forskolin-Sepharose was synthesized by the method of Pfeuffer et al.(1985).Adenylate Cyclase Assay. Adenylate cyclase was assayed by the general method of Salomon et al.(1979) using [a-32P] ATP as a substrate and [3H] cAMP to monitor product recovery. Assays contained the following in a final volume of 250 mL: 20 mM Tris-HCl, pH 7.5, 1
