Engineering a living biomaterial via bacterial surface capture of environmental molecules
Engineering a living biomaterial via bacterial surface capture of environmental molecules
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DOI：
10.1093/synbio/ysy017
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发表时间：
2018-09
影响因子：
3.2
通讯作者：
F. Y. Scott;K. Heyde;MaryJoe K. Rice;Warren C. Ruder
中科院分区：
文献类型：
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作者：
F. Y. Scott;K. Heyde;MaryJoe K. Rice;Warren C. Ruder
合成生物学在生物材料科学中具有重要的潜力，因为合成工程细胞可以产生新的生物材料，或者可以作为新生物材料的活性成分。在这里，我们描述了一种新的生物材料的创建，采用工程表面显示与环境相互作用的活细菌成分。我们首先开发了一种基因构建体，该基因构建体能够同时表达胞质mCherry和能够与苄基鸟嘌呤（BG）基团共价结合的表面展示的催化活性酶。然后，我们使用这些基因工程细胞在微流体通道内创建了一种功能性生物材料。当工程化细胞在诱导后共价结合到周围BG修饰的分子时，材料形成。考虑到广泛的材料适用于BG-基团的功能化，我们的系统提供了一个概念验证的BG-功能化的分子的螯合和组装上的流体扫过，活的生物材料表面。
Abstract Synthetic biology holds significant potential in biomaterials science as synthetically engineered cells can produce new biomaterials, or alternately, can function as living components of new biomaterials. Here, we describe the creation of a new biomaterial that incorporates living bacterial constituents that interact with their environment using engineered surface display. We first developed a gene construct that enabled simultaneous expression of cytosolic mCherry and a surface-displayed, catalytically active enzyme capable of covalently bonding with benzylguanine (BG) groups. We then created a functional living material within a microfluidic channel using these genetically engineered cells. The material forms when engineered cells covalently bond to ambient BG-modified molecules upon induction. Given the wide range of materials amenable to functionalization with BG-groups, our system provides a proof-of-concept for the sequestration and assembly of BG-functionalized molecules on a fluid-swept, living biomaterial surface.