Microorganisms from composting leaves: Ability to produce extracellular degradative enzymes
Microorganisms from composting leaves: Ability to produce extracellular degradative enzymes
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堆肥叶子中的微生物：产生细胞外降解酶的能力
DOI：
10.1007/bf02011649
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发表时间：
1975
影响因子：
3.6
通讯作者：
S. Anagnostakis
中科院分区：
文献类型：
--
作者：
L. Hankin;R. P. Poincelot;S. Anagnostakis
混合种群的细菌，真菌和放线菌在叶堆肥堆进行了检查超过100天的测试期间，他们的能力，产生细胞外的蛋白水解，脂肪分解，淀粉分解，纤维素分解，果胶分解，尿素分解酶和能力，利用烷烃。尿素被添加到叶片中，以调节碳氮比，但在保持适当的比例是没有价值的，因为它是在最初几天内降解。微生物分泌的降解酶与堆体温度有关。在许多情况下，能够在中等温度下产生特定胞外酶的生物体能够在高温下生长，但要么不分泌特定酶，要么酶被高温灭活。
Mixed populations of bacteria, fungi, and actinomycetes in a leaf compost pile were examined over a 100-day test period for their ability to produce extracellular proteolytic, lipolytic, amylolytic, cellulolytic, pectolytic, and ureolytic enzymes and ability to utilize alkanes. Urea was added to the leaves to adjust the carbon to nitrogen ratio but was of little value in maintaining the proper ratio since it was degraded within the first few days. The degradative enzymes excreted by microorganisms was dependent on the temperature of the pile. In many cases organisms able to produce specific extracellular enzymes at medium temperatures were able to grow at high temperatures, but either did not excrete the specific enzymes or the enzymes were inactivated by the high temperature.