SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators1
SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators1
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SlREM1 通过激活氧化爆发和其他调节因子来触发细胞死亡。
DOI：
10.1104/pp.20.00120
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发表时间：
2020-06-01
期刊：
影响因子：
7.4
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
--
作者：
Cai, Jianghua;Chen, Tong;Tian, Shiping
程序性细胞死亡（PCD）是植物免疫反应的一个高度调节的特征，涉及多个分子参与者。Remorins（REMs）是植物特有的蛋白质，具有多种生物学功能，但其在PCD和植物防御中的作用尚不清楚。在这里，我们报告的作用，在抗病性，免疫反应和PCD的调节。番茄REMORIN 1（SlREM 1）的过表达增加了番茄对灰葡萄孢的敏感性，并且该基因的异源表达触发了本氏烟草叶片中的细胞死亡。进一步的研究表明，SlREM 1的173至187位氨基酸和磷酸化在SlREM 1触发的细胞死亡中起关键作用。有趣的是，多个番茄REM诱导细胞死亡在N. benthamianaleae。酵母双杂交，分裂荧光素酶互补，和免疫共沉淀试验都表明，resistin蛋白可以形成同源和杂合物。使用相对和绝对定量蛋白质组学的同量异序标签，我们确定了一些与细胞死亡调控相关的蛋白质，以及本氏烟草爆发氧化酶同源物B（这是活性氧产生所必需的），在表达SlREM 1的叶片中显著上调。SlREM 1的异源表达增加了活性氧的积累，并触发了其他细胞死亡调节因子。研究结果表明，SlREM 1是植物细胞死亡的正向调节因子，为理解植物PCD分子调控网络提供了线索;番茄红素蛋白通过增加活性氧和其他防御因子的积累导致细胞死亡。
Programmed cell death (PCD), a highly regulated feature of the plant immune response, involves multiple molecular players. Remorins (REMs) are plant-specific proteins with varied biological functions, but their function in PCD and plant defense remains largely unknown. Here, we report a role for remorin in disease resistance, immune response, and PCD regulation. Overexpression of tomato (Solanum lycopersicum)REMORIN1(SlREM1) increased susceptibility of tomato to the necrotrophic fungusBotrytis cinereaand heterologous expression of this gene triggered cell death inNicotiana benthamianaleaves. Further investigation indicated that amino acids 173 to 187 and phosphorylation of SlREM1 played key roles in SlREM1-triggered cell death. Intriguingly, multiple tomato REMs induced cell death inN.benthamianaleaves. Yeast two-hybrid, split luciferase complementation, and coimmunoprecipitation assays all demonstrated that remorin proteins could form homo- and heterocomplexes. Using isobaric tags for relative and absolute quantitative proteomics, we identified that some proteins related to cell death regulation, as well asN.benthamianaRESPIRATORY BURST OXIDASE HOMOLOG B (which is essential for reactive oxygen species production), were notably upregulated inSlREM1-expressing leaves. Heterologous expression ofSlREM1increased reactive oxygen species accumulation and triggered other cell death regulators. Our findings indicate that SlREM1 is a positive regulator of plant cell death and provide clues for understanding the PCD molecular regulatory network in plants.A tomato remorin protein causes cell death by increasing the accumulation of reactive oxygen species and other defense factors.