SEQUENCE-BASED DIFFERENTIATION OF STRAINS IN THE MYCOBACTERIUM-AVIUM COMPLEX
SEQUENCE-BASED DIFFERENTIATION OF STRAINS IN THE MYCOBACTERIUM-AVIUM COMPLEX
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DOI：
10.1128/jb.175.10.2818-2825.1993
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发表时间：
1993-05-01
影响因子：
3.2
通讯作者：
WILSON, KH
中科院分区：
文献类型：
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作者：
FROTHINGHAM, R;WILSON, KH
测定了35株鸟分枝杆菌复合群参考菌株16S-23SrDNA内转录间隔区（ITS）序列。获得了12个不同的ITS序列，每个序列都定义了一个“序列”;序列由具有特定序列的菌株组成。ITS序列与M.禽分枝杆菌（16株，4个ITS序列）和胞内分枝杆菌（12株，1个ITS序列）。其他七个M。鸟型复合群菌株的ITS序列与M. avium和M.细胞内和彼此。16S-23SrDNAITS序列比16SrDNAITS序列具有更高的变异性，广泛应用于种属鉴定。基于ITS的系统发育树与基于16S rDNA的系统发育树一致，但更为详细，分支较长。ITS序列测定结果与M. avium和M.细胞内探针（Gen-Probe）检测31个菌株中的30个。血清学检测与ITS测序相关性较差。序列相同的菌株属于不同的血清型，同一血清型的菌株序列不同。16S-23S rDNA ITS序列的测定对于多种细菌的种和菌株的区分是有用的，并且应该适用于流行病学、诊断、毒力和分类学的研究。
The complete 16S-23S rDNA internal transcribed spacer (ITS) was sequenced in 35 reference strains of the Mycobacterium avium complex. Twelve distinct ITS sequences were obtained, each of which defined a ''sequevar''; a sequevar consists of the strain or strains which have a particular sequence. ITS sequences were identified which corresponded to M. avium (16 strains, four ITS sequevars) and Mycobacterium intracellulare (12 strains, one ITS sequevars). The other seven M. avium complex strains had ITS sequences which varied greatly from those of M. avium and M. intracellulare and from each other. The 16S-23S rDNA ITS was much more variable than 16S rDNA, which is widely used for genus and species identification. Phylogenetic trees based on the ITS were compatible with those based on 16S rDNA but were more detailed and had longer branches. The results of ITS sequencing were consistent with the results of hybridization with M. avium and M. intracellulare probes (Gen-Probe) for 30 of 31 strains tested. Serologic testing correlated poorly with ITS sequencing. Strains with the same sequence were different serovars, and those of the same serovar had different sequences. Sequencing of the 16S-23S rDNA ITS should be useful for species and strain differentiation for a mide variety of bacteria and should be applicable to studies of epidemiology, diagnosis, virulence, and taxonomy.