The inheritance of seed resistance to Callosobruchus maculatus F. in cowpea (Vigna unguiculata L. Walp.). I. Analyses of parental, F1, F2, F3 and backcross seed generations
The inheritance of seed resistance to Callosobruchus maculatus F. in cowpea (Vigna unguiculata L. Walp.). I. Analyses of parental, F1, F2, F3 and backcross seed generations
复制标题
豇豆 (Vigna unguiculata L. Walp.) 种子对 Callosobruchus maculatus F. 的抗性遗传。
DOI：
10.1071/ar9830681
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
A. Gatehouse
中科院分区：
文献类型：
--
作者：
R. Redden;P. Dobie;A. Gatehouse
对亲本、杂种 F1、F2、F3 和易感豇豆品系与抗性种质 TVu 2027 之间五次杂交的回交种子代进行了豇豆种子上豆象出苗的生物测定。用互惠 F1 杂种种子和互惠回交杂种种子重复了该研究。对于后者，还测量了豆象羽化的平均天数。还对来自一次互交的单个 F2 植物的 F3 种子进行了生物测定。对亲本、相互 F1 代以及相互和非相互 F3 种子代进行胰蛋白酶抑制剂测定。结果表明：（1）耐药性的表达主要由母源基因型决定； (2)抗性可能作为主要基因效应遗传，具有抗性隐性，并且在不同杂交中存在不同效应的修饰基因； (3) 细胞质效应似乎不存在； (4)在某种回交组合中可以检测到父本或胚胎基因型对种子抗性的影响； (5)胰蛋白酶抑制剂与抗性的表达部分相关，但与F2分离不相关，并且似乎是定量遗传的并且在杂交之间可变。因此，迄今为止尚未发现的其他机制也可能与种子对豆象的抗性的表达有关。
Bioassays for bruchid emergence on cowpea seeds were conducted on the parent, hybrid F1, F2, F3, and backcross seed generations of five crosses between susceptible cowpea lines and the resistant accession TVu 2027. The study was repeated with reciprocal F1 hybrid seeds, and reciprocal backcross hybrid seeds. For the latter, the mean day of bruchid emergence was also measured. F3 seed from individual F2 plants of one reciprocal cross were also bioassayed. Trypsin inhibitor determinations were made on the parental, reciprocal F1 and reciprocal and non-reciprocal F3 seed generations. The results indicated: (1) that the expression of resistance is mainly determined by the maternal genotype; (2) that resistance may be inherited as a major gene effect, with resistance recessive, and with the presence of modifier genes of different effects in different crosses; (3) that a cytoplasmic effect appears to be absent; (4) that a paternal or embryo genotypic effect on seed resistance can be detected in a certain backcross combination; (5) that trypsin inhibitors are partly associated with expression of resistance, but are uncorrelated in F2 segregation, and appear to be quantitatively inherited and variable between crosses. Thus additional mechanisms so far undetected may also be implicated in the expression of seed resistance to bruchids.