Patch and whole-cell voltage clamp of single mammalian visceral and vascular smooth muscle cells
Patch and whole-cell voltage clamp of single mammalian visceral and vascular smooth muscle cells
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单个哺乳动物内脏和血管平滑肌细胞的贴片和全细胞电压钳
DOI：
10.1007/bf01970006
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
C. Benham
中科院分区：
文献类型：
--
作者：
T. Bolton;R. Lang;T. Takewaki;C. Benham
分散的哺乳动物内脏和血管平滑肌的组成细胞已允许记录下全细胞电压钳膜电流，和电流通过单个离子通道使用膜片钳技术。在正常生理溶液中，在电压钳下对单个细胞施加矩形去极化步骤产生净内向电流，随后产生净外向电流。在孤立的，“由内而外”补丁的细胞膜的钙和电位敏感的K通道（100 pS电导）和钙不敏感，电位敏感的K+通道（50 pS电导）与缓慢的动力学迄今已被确定和表征。
Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, ‘inside-out’ patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K+ channel (50 pS conductance) with slow kinetics have so far been identified and characterized.
单个新鲜分离的平滑肌细胞膜的被动特性。
DOI：
10.1152/ajpcell.1980.239.5.c153
发表时间：
1980
期刊：
The American journal of physiology
影响因子：
--
作者：
Singer,JJ;WalshJr,JV
通讯作者：
WalshJr,JV