Structure and expression of TIS21, a primary response gene induced by growth factors and tumor promoters.
Structure and expression of TIS21, a primary response gene induced by growth factors and tumor promoters.
复制标题
DOI：
10.1016/s0021-9258(18)98716-8
复制
发表时间：
1991-08
期刊：
影响因子：
--
通讯作者：
B. Fletcher;R. Lim;B. Varnum;D. Kujubu;R. Koski;H. Herschman
中科院分区：
文献类型：
--
作者：
B. Fletcher;R. Lim;B. Varnum;D. Kujubu;R. Koski;H. Herschman
TIS21 基因是一种初级反应基因，在 3T3 细胞中由肿瘤启动子和促细胞分裂剂十四烷酰佛波醇乙酸酯快速、短暂地诱导。 TIS21 cDNA 的预测开放阅读框编码 158 个氨基酸的蛋白质，与任何已知蛋白质没有明显的相似性。针对大肠杆菌中产生的 TIS21 重组蛋白制备的抗血清可从 Swiss 3T3 细胞中沉淀出 17-kDa 的蛋白。 cDNA 的 2040 个核苷酸的 3'-非翻译区包含一个不寻常的 T18 序列。 TIS21 基因有一个 1.4 KB 的内含子，它中断了开放阅读框，并且在其他方面与 cDNA 序列相同。 TIS21 基因的 5' 侧翼序列包含 TATA 和 CAAT 盒型序列、三个潜在的 Sp1 位点、两个假定的环 AMP 响应元件、两个潜在的 AP1 结合元件和一个 AP2 元件。可能的 Z-DNA 结构包含 29 个 AC 重复序列，存在于转录起始点 660 个核苷酸处。尽管不存在共有的血清反应元件，但含有 TIS21 启动子的 460 个核苷酸片段的荧光素酶报告构建体的表达由乙酸十四烷酰佛波醇、毛喉素、表皮生长因子和血清诱导。
The TIS21 gene is a primary response gene that is induced rapidly and transiently in 3T3 cells by the tumor promoter and mitogen tetradecanoyl phorbol acetate. The predicted open reading frame of the TIS21 cDNA encodes a protein of 158 amino acids with no obvious similarity to any known protein. Antiserum prepared to TIS21 recombinant protein produced in Escherichia coli precipitates a 17-kDa protein from Swiss 3T3 cells. The 2040-nucleotide 3‘-untranslated region of the cDNA includes an unusual T18 sequence. The TIS21 gene has a single 1.4-kilobase intron which interrupts the open reading frame and is otherwise identical to the cDNA sequence. The 5‘-flanking sequence of the TIS21 gene contains TATA and CAAT box-type sequences, three potential Sp1 sites, two putative cyclic AMP response elements, two potential AP1 binding elements, and an AP2 element. A possible Z-DNA structure of 29 AC repeats is present 660 nucleotides from the start of transcription. Expression from a luciferase reporter construct containing a 460-nucleotide fragment of the TIS21 promoter is induced by tetradecanoyl phorbol acetate, forskolin, epidermal growth factor, and serum, despite the absence of a consensus serum response element.