Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications
Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications
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DOI：
10.1016/j.micinf.2010.10.001
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发表时间：
2011-01-01
影响因子：
5.8
通讯作者：
Akari, Hirofumi
中科院分区：
文献类型：
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作者：
Saito, Akatsuki;Nomaguchi, Masako;Akari, Hirofumi
人类免疫缺陷病毒1型（HIV-1）在旧大陆猴子中几乎不复制。最近，一种HIV-1突变克隆NL-DT5R被证明能够在长尾猴中复制，但不能在恒河猴（RM）中复制，其中一小部分gag和整个vif基因被sivmac239衍生的基因所取代。在本研究中，我们发现，与NL-DT5R相比，修饰的嗜猴HIV-1 (HIV-1mt) MN4-5S在食蟹猴中获得了高效复制的能力，而NL-DT5R和MN4-5S在RM细胞中都无法复制。这些结果表明，多种决定因素可能参与了猕猴体内HIV-1复制的限制，这取决于猕猴的种类。新的HIV-1mt克隆将有助于研究抗病毒宿主因子调控HIV-1在猕猴体内复制的分子机制。(C) 2010巴斯德研究所。Elsevier Masson SAS出版。版权所有。
Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.