The protective effect of the ketogenic diet on traumatic brain injury-induced cell death in juvenile rats
The protective effect of the ketogenic diet on traumatic brain injury-induced cell death in juvenile rats
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DOI：
10.1080/02699050902788469
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发表时间：
2009-01-01
期刊：
影响因子：
1.9
通讯作者：
Yin, Hong-Xia
中科院分区：
文献类型：
--
作者：
Hu, Zhi-Gang;Wang, Han-Dong;Yin, Hong-Xia
背景：生酮饮食（KD）是治疗顽固性癫痫的有效方法，尤其是在儿科人群中，越来越多的研究表明生酮饮食具有神经保护作用。然而，很少有研究关注 KD 在创伤性脑损伤 (TBI) 中的神经保护作用。本研究旨在探讨 KD 对 TBI 的影响。方法和步骤：将雄性Sprague-Dawley大鼠（n = 60）根据饮食（KD与正常饮食）和脑部是否损伤随机分为四组。 TBI 是使用 Feeney 重量下降模型生成的。通过湿重/干重比来估计脑水肿； Bax 和 Bcl-2 mRNA 水平通过 RealTime-PCR 测定；通过Western blot检测Bax和Bcl-2蛋白水平。此外，使用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记 (TUNEL) 方法检查半影区域的细胞凋亡。主要结局和结果：结果表明，Bax mRNA 和蛋白质水平在 TBI 后 72 小时显着升高，并通过 KD 给药而降低。 TBI 和 KD 都不影响 Bcl-2 mRNA 和蛋白质水平。 KD 给药还可以减少脑水肿和细胞凋亡。结论：这些结果表明，KD 可能是治疗患有 TBI 后果的儿童的有效方法。
Background: The ketogenic diet (the KD) is an effective treatment for intractable epilepsy, especially in the paediatric population, and a growing number of studies have shown the neuroprotective role of the KD. However, few studies focused on the neuroprotective effects of the KD in traumatic brain injury (TBI). The present study aimed to investigate the effects of the KD on TBI. Methods and procedures: Male Sprague-Dawley rats (n = 60) were randomly divided into four groups according to the diet fed (the KD vs normal diet) and whether brain was injured or not. TBI was produced using Feeney weight drop model. Brain oedema was estimated by wet/dry weight ratio; Bax and Bcl-2 mRNA levels were determined by RealTime-PCR; Bax and Bcl-2 protein levels were detected by Western blot. Furthermore, cellular apoptosis in the penumbra area was examined using terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) method. Main outcomes and results: The results indicated that both Bax mRNA and protein levels were significantly elevated 72 hours after TBI and decreased by KD administration. Neither TBI nor the KD affected Bcl-2 mRNA and protein levels. KD administration also reduced brain oedema and cellular apoptosis. Conclusion: These results suggest that the KD might be a useful treatment for children suffering from the consequences of TBI.