Low-energy ion beam-induced mutation in Thai jasmine rice (Oryza sativa L. cv. KDML 105)
Low-energy ion beam-induced mutation in Thai jasmine rice (Oryza sativa L. cv. KDML 105)
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DOI：
10.1016/j.surfcoat.2006.02.057
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发表时间：
2007-08
影响因子：
5.4
通讯作者：
B. Phanchaisri;R. Chandet;L. D. Yu;T. Vilaithong;S. Jamjod;S. Anuntalabhochai
中科院分区：
文献类型：
--
作者：
B. Phanchaisri;R. Chandet;L. D. Yu;T. Vilaithong;S. Jamjod;S. Anuntalabhochai
选用能量为60~125keV、剂量为1×1016~5×1017ion/cm2的低能离子束对泰国茉莉花水稻进行诱变处理。KDML 105)在清迈大学。对其中一个命名为BKOS6的水稻突变体进行了鉴定。用能量为60keV、离子注量为2×1016ion/cm2的N~++N~+~+离子轰击KDML_(105)水稻胚获得突变体。BKOS6的表型变异表现为矮小，叶鞘、叶颈、叶耳、叶舌呈红色/紫色，叶片、种皮和果皮上有深棕色条纹。突变体的繁殖期为反季节栽培(3-7月)。采用HAT-RAPD技术对突变体的基因组变异进行分析。在10个引物中，有两个在450和400个碱基上检测到另外两条DNA条带。DNA测序结果表明，450hp和400hp片段分别与水稻黄烷类3‘羟基酶和细胞色素P450的氨基酸序列有60%和61%的同源性。
Low-energy ion beam bombardment at energy levels in the range of 60–125 keV and ion fluences (dose) of 1×1016–5×1017ions/cm2was chosen for mutation induction in Thai jasmine rice (Oryza sativa L. cv. KDML 105) at Chiang Mai University. One of the rice mutants designated BKOS6 was characterized. The rice mutant was obtained from KDML 105 rice embryos bombarded with N++N2+ions at an energy level of 60 keV and ion fluence of 2×1016ions/cm2. Phenotypic variations of BKOS6 were short in stature, red/purple color in leaf sheath, collar, auricles, ligule, and dark brown stripes on leaf blade, dark brown seed coat and pericarp. The mutant's reproductive stage was found in off-season cultivation (March–July). HAT-RAPD (High Annealing Temperature-Random Amplified Polymorphic DNA) was applied for analysis of genomic variation in the mutant. Of 10 primers, two primers detected two additional DNA bands at 450 bp and 400 bp. DNA sequencing revealed that the 450 bp and the 400 bp fragments were 60% and 61% identity to amino acid sequence of flavanoid 3′hydroxylase and cytochrome P450 of O. sativa japonica, respectively.