Benzo[a]pyrene effects on reproductive endpoints in Fundulus heteroclitus.
Benzo[a]pyrene effects on reproductive endpoints in Fundulus heteroclitus.
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DOI：
10.1093/toxsci/kfu064
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发表时间：
2014-07
期刊：
影响因子：
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通讯作者：
Frank Booc;C. Thornton;A. Lister;D. Maclatchy;K. Willett
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作者：
Frank Booc;C. Thornton;A. Lister;D. Maclatchy;K. Willett
苯并[a]芘（BaP）是一种多环芳烃（PAH），参与调节芳香酶的功能，可能会中断正常的生殖功能。本研究的目的是使用鱼类模型，底heteroclitus，以评估苯并（a）芘暴露是否会对生殖产生不利影响。成鱼暴露于水中苯并（a）芘标称浓度（0、1或10 μg/l）28天。在暴露的后半部分（第14-28天）将雄性和雌性动物合并，以定量产卵和受精成功。高剂量的BaP显著降低了F1胚胎的受精率和随后的孵化成功率。在雄性中，与对照组相比，10 μg/l BaP显著降低了性腺重量和血浆睾酮浓度。睾丸的组织学检查，包括精原细胞，精母细胞和精子细胞囊肿面积，每个阶段的囊肿百分比，和每个曲细精管的精子面积没有显着影响。其他生物标志物，包括雄性肝脏重量，肝脏卵黄蛋白原（vtg）mRNA表达和精子浓度，也没有受到影响。在女性中，雌二醇浓度显着降低后，苯并（a）芘曝光，但产蛋量，性腺重量，肝脏重量，vtg表达和卵母细胞成熟没有改变。暴露的父母在1和3周postthatch底幼虫的类固醇浓度没有显着变化。在这些环境相关浓度的苯并（a）芘暴露引起负面改变，特别是在雄鱼中，与生殖和多代胚胎存活相关的生物化学和表型生物标志物。
Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that has been implicated in modulating aromatase enzyme function with the potential to interrupt normal reproductive function. The aim of this study was to use a fish model, Fundulus heteroclitus, to assess whether BaP exposure adversely impacts reproduction. Adult fish were exposed to waterborne BaP nominal concentrations of (0, 1, or 10 μg/l) for 28 days. Males and females were combined for the second half of the exposure (days 14-28) in order to quantitate egg production and fertilization success. Egg fertilization and subsequent hatching success of F1 embryos was significantly decreased by the high dose of BaP. In males, both gonad weight and plasma testosterone concentrations were significantly reduced compared to controls by 10 μg/l BaP. Histopathological examination of testes including spermatogonia, spermatocyte and spermatid cyst areas, percentage of cysts per phase, and area of spermatozoa per seminiferous tubule were not significantly affected. Other biomarkers, including male liver weight, liver vitellogenin (vtg) mRNA expression and sperm concentrations, were also not affected. In females, estradiol concentrations were significantly reduced after BaP exposure, but egg production, gonad weight, liver weight, vtg expression and oocyte maturation were not altered. Steroid concentrations in Fundulus larvae from exposed parents at 1 and 3 weeks posthatch were not significantly changed. BaP exposure at these environmentally relevant concentrations caused negative alterations particularly in male fish to both biochemical and phenotypic biomarkers associated with reproduction and multigenerational embryo survival.