Using centrosome fragments in the directed assembly of microtubules.
Using centrosome fragments in the directed assembly of microtubules.
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在微管的定向组装中使用中心体片段。
DOI：
10.1166/jnn.2009.c043
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发表时间：
2009
影响因子：
--
通讯作者：
Siegel,RichardW
中科院分区：
文献类型：
--
作者：
Shang,Wen;Crone,DonnaE;Yang,Hoichang;Dordick,JonathanS;Palazzo,RobertE;Siegel,RichardW
在动物细胞中，微管由中心体组成，中心体是直径1-2 μm的细胞器。沿着的是通过超声处理在体外产生功能性中心体片段，并由图案化的中心体片段指导微管组装。虽然中心体碎片比完整的中心体小，但它们的微管组织功能仍然保留。与完整的中心体相比，中心体片段模板在微管的图案化和组装中提供了更大的灵活性和更好的覆盖率。这项工作提供了理由和潜力的大面积组装的微管，并应扩大应用中心体和中心体组件的创建微管为基础的纳米级器件。
In animal cells, microtubules are organized by centrosomes, which are 1–2 μm diameter organelles. The generation of functional centrosome fragments in-vitro through ultrasonication is presented along with microtubule assembly directed by the patterned centrosome fragments. While centrosome fragments are smaller than the fully constituted centrosomes, their microtubule organization function is retained. The centrosome fragment templates offer greater flexibility and better coverage in both patterning and assembly of microtubules when compared with intact centrosomes. This work provides the rationale and potential for the large-area assembly of microtubules and should expand the application of centrosomes and centrosome components for the creation of microtubule-based nanoscale devices.