Alveolar macrophages have greater amounts of the enzyme 5-lipoxygenase than do monocytes.
Alveolar macrophages have greater amounts of the enzyme 5-lipoxygenase than do monocytes.
复制标题
肺泡巨噬细胞比单核细胞含有更多的 5-脂氧合酶。
DOI：
10.1152/jappl.1992.73.2.781
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hunninghake,GW
中科院分区：
文献类型：
--
作者：
Pueringer,RJ;Bahns,CC;Hunninghake,GW
在A23187刺激后，肺泡巨噬细胞比血液单核细胞释放更大量的白三烯B4（LTB 4）和5-羟基二十碳四烯酸（5-HETE）。肺泡巨噬细胞中5-脂氧合酶活性增强的机制尚不清楚。在这些研究中，我们确定了肺泡巨噬细胞是否比血液单核细胞具有更大量的5-脂氧合酶。我们证实，肺泡巨噬细胞释放更多的LTB 4 A23187刺激后比同等数量的血液单核细胞。在A23187的存在和不存在下，肺泡巨噬细胞有更大量的免疫反应性5-脂氧合酶，通过Western分析确定，在每个细胞和每个蛋白质的基础上比血液单核细胞。细胞中5-脂氧合酶的量大致与两种细胞释放的LTB 4的量相关。这些观察结果表明，A23187刺激肺泡巨噬细胞释放比血液单核细胞更大量的LTB 4和5-HETE，部分原因是由于更大量的5-脂氧合酶。
Alveolar macrophages release greater amounts of leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) after A23187 stimulation than do blood monocytes. The mechanisms for this enhanced 5-lipoxygenase activity in alveolar macrophages are unknown. In these studies, we determined whether alveolar macrophages have greater amounts of the enzyme 5-lipoxygenase than do blood monocytes. We confirmed that alveolar macrophages released greater amounts of LTB4 after A23187 stimulation than did equivalent numbers of blood monocytes. In both the presence and absence of A23187, alveolar macrophages had greater amounts of immunoreactive 5-lipoxygenase, determined by Western analysis, on a per cell and a per protein basis than did blood monocytes. The amounts of 5-lipoxygenase enzyme in the cells roughly correlated with the amounts of LTB4 released by both types of cells. These observations suggest that A23187 stimulates alveolar macrophages to release greater amounts of LTB4 and 5-HETE than blood monocytes, in part, due to the greater amounts of 5-lipoxygenase.