Comparison of cryotherapy and nephrectomy in treating implanted VX-2 carcinoma in rabbit kidneys
Comparison of cryotherapy and nephrectomy in treating implanted VX-2 carcinoma in rabbit kidneys
复制标题
DOI：
10.1111/j.1464-410x.2004.05015.x
复制
发表时间：
2004-09-01
影响因子：
4.5
通讯作者：
Lee, FT
中科院分区：
文献类型：
--
作者：
Nakada, SY;Jerde, TJ;Lee, FT
为比较冷冻消融术和根治性肾切除术治疗兔肾移植性VX-2肿瘤的疗效，对27只新西兰白色兔进行了初步研究，以评估VX-2肿瘤的自然病程。将VX-2肿瘤节段（1 mm（3））从携带者家兔的皮下肿瘤中切除，并植入研究家兔的左肾中。在植入后，允许肿瘤生长6、7、8、9、10、14、18、21和28天。分析肾脏和周围浆膜中的肿瘤进展，并确定肺、肝、脾和膀胱中的转移性肿瘤存在。之后，将80只家兔在下极植入VX-2，并随机分为三组：第1组，35只家兔在VX-2植入后7天使用15分钟双重冷冻技术和-20 ℃的5 mm肿瘤边缘目标温度进行冷冻消融治疗;第2组，35只家兔接受开放性根治性肾切除术治疗;或第3组，10只未治疗对照组。将兔子饲养22天后处死，取出肾脏、肺、肝脏、脾脏、膀胱和输尿管，进行大体和组织学检查，以确定肿瘤的存在。在初步研究中，肿瘤生长7天后始终为50-100 mm 3，没有转移性疾病的迹象。到第10天，平均肾脏肿瘤为230 mm（3），三只兔子中有两只在肺中发生转移。到第18天，平均原发肿瘤为5504 mm（3），所有兔子都有转移性疾病。在对比研究中，冷冻治疗治愈了66%的移植兔，而肾切除术治愈了64%。所有未治疗的对照组均具有显著的局部和转移性肿瘤。在处理后15天处死的动物中，两个处理组和未处理对照组之间的结果显示出显著差异（P < 0.002）。冷冻治疗和开放性肾切除术组之间无病生存率无差异（P = 0.78）。与未治疗对照组相比，冷冻消融和根治性肾切除术治疗植入的VX-2肾肿瘤均有效。冷冻消融术和肾切除术之间没有统计学显著差异。
To compare the efficacy of cryoablation and radical nephrectomy for treating implanted VX-2 carcinoma in rabbit kidneys.A pilot study was conducted using 27 New Zealand white rabbits to assess the natural history of VX-2 carcinoma in the kidney. VX-2 tumour segments (1 mm(3)) were excised from subcutaneous tumours in carrier rabbits and implanted in the left kidney of study rabbits. The tumours were allowed to grow for 6, 7, 8, 9, 10, 14, 18, 21 and 28 days after implantation. Tumour progression was analysed in the kidney and surrounding serosa, and metastatic tumour presence in the lung, liver, spleen and bladder determined. After this, 80 rabbits were implanted with VX-2 in the lower pole and randomized into one of three groups: group 1, 35 rabbits treated 7 days after VX-2 implantation with cryoablation using a 15-min double-freeze technique and a 5-mm tumour margin target temperature of - 20 degreesC; group 2, 35 rabbits treated with open radical nephrectomy; or group 3, 10 untreated controls. Rabbits were maintained for 22 days and then killed; the kidneys, lungs, liver, spleen, urinary bladder and ureter were removed, and examined grossly and histologically for tumour presence.In the pilot study there was a consistent 50-100 mm(3) tumour after 7 days of growth, with no evidence of metastatic disease. By day 10 the mean kidney tumour was 230 mm(3) and two of three rabbits had metastases in the lung. By day 18 the mean primary tumour was 5504 mm(3) and all rabbits had metastatic disease. In the comparison study, cryotherapy cured 66% of implanted rabbits while nephrectomy cured 64%. All untreated controls had significant local and metastatic tumours. Findings in animals killed at 15 days after treatment showed significant differences between both treatment groups and untreated controls (P < 0.002). There was no difference in disease-free survival between the cryotherapy and open nephrectomy groups (P = 0.78)Both cryoablation and radical nephrectomy were effective in treating implanted VX-2 renal tumours compared with untreated controls. There was no statistically significant difference between cryoablation and nephrectomy.