Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended-spectrum cephalosporinase-producing Escherichia coli in slaughter pigs
Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended-spectrum cephalosporinase-producing Escherichia coli in slaughter pigs
复制标题
DOI：
10.1093/jac/dks427
复制
发表时间：
2013-03-01
影响因子：
5.2
通讯作者：
Aarestrup, Frank M.
中科院分区：
文献类型：
--
作者：
Agerso, Yvonne;Aarestrup, Frank M.
为了衡量在丹麦养猪业中自愿禁止头孢菌素使用对猪和猪肉中产生超广谱头孢菌素酶(ESC)的大肠杆菌流行率的影响。为了检测产生ESC的大肠杆菌，包括三种抽样类型：屠宰时，在头孢菌素自愿禁令生效之前的2009年和2010年6月以及实施头孢菌素自愿禁令之后的两个时期(2010年7月)从猪只身上采集盲肠样本；在养殖场层面，在2010年和2011年从不同猪圈收集了五个粪便样本；以及在2009年至2011年期间在零售店和销售点随机收集猪肉样本。用1 mg/L的头孢曲松对麦康基发酵液进行选择性浓缩，分离到产ESC的大肠埃希菌。用聚合酶链式反应、微管芯片和测序技术检测ESC基因，从2010年7月开始，头孢菌素类药物的使用量几乎为零。生猪屠宰时产ESC大肠埃希氏菌的发生率在2009年[10.8(85/786)]和2010年[11.8(48/407)]之间无显著差异(P0.7)，但在2011年[3.6(28/777)]显著下降(P0.001)。从2010年[11(11/99)]到2011年(0/78)，养猪场水平产生ESC的大肠杆菌的发生率也显著下降(P0.002)。以bla(CTX-M-1)基因最常见(63例)，其次为bla(CTX-M-14)和bla(CTX-M-15)。在猪肉中的发生率在1.3到0.9之间。停止在养猪业中使用已经很低的头孢菌素，大大减少了产生ESC的大肠杆菌的发生。
To measure the effect of a voluntary ban on cephalosporin usage in the Danish pig production on the prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in pigs and pork.Data on cephalosporin consumption were obtained from the VetStat database. For detection of ESC-producing E. coli, three sampling types were included: at slaughter, caecal samples were collected from pigs in 2009 and 2010 (June) before and in two periods (2010 and 2011) after a voluntary ban on cephalosporins was effected (July 2010); at farm level, pools of five stool samples from different pigsties were collected in 2010 and in 2011; and samples from pork were collected randomly at retail stores and outlets from 2009 to 2011. ESC-producing E. coli was isolated after selective enrichment in MacConkey broth with 1 mg/L ceftriaxone. ESC genes were detected using PCR, microtube array and sequencing.From July 2010 the consumption of cephalosporins approximated zero. The occurrence of ESC-producing E. coli in pigs at slaughter was not significantly different (P0.7) between 2009 [10.8 (85/786)] and 2010 [11.8 (48/407)], but in 2011 the occurrence [3.6 (28/777)] decreased significantly (P0.001). A significant decrease (P0.002) in occurrence of ESC-producing E. coli at pig farm level from 2010 [11 (11/99)] to 2011 (0/78) was also observed. The bla(CTX-M-1) gene was most often detected (63), but bla(CTX-M-14) and bla(CTX-M-15) were also found. Occurrence in pork was between 1.3 and 0.9.The discontinuation of an already low use of cephalosporins in pig production has significantly reduced the occurrence of ESC-producing E. coli.