Preparation of an autologous heart valve with a stent (stent-biovalve) using the stent eversion method
Preparation of an autologous heart valve with a stent (stent-biovalve) using the stent eversion method
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DOI：
10.1002/jbm.b.33086
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发表时间：
2014-07-01
影响因子：
3.4
通讯作者：
Nakayama, Yasuhide
中科院分区：
文献类型：
--
作者：
Mizuno, Takeshi;Takewa, Yoshiaki;Nakayama, Yasuhide
我们设计了一种用支架构建自体心脏瓣膜的新方法，称为支架生物瓣膜。在使用体内组织构建技术构建完全自体心脏瓣膜（生物瓣膜）时，小叶组织通过向内生长进入制备模具的狭窄孔中形成，经常导致生物瓣膜制备延迟或不完整。在该技术中，将旋转后的自膨胀镍钛诺支架安装在丙烯酸柱状部件上，并部分覆盖有三个狭缝的丙烯酸圆柱形部件。将这种组装好的模具放入比格犬或山羊的皮下腹腔袋中4周。将被包膜组织收获和修剪后，去除丙烯酸部分，得到一个管状中空结构，膜性组织的三个口袋瓣刚性固定在支架的外表面。然后，将支架从里面翻转到原来的形状，从而将口袋皮瓣从外面移动到里面。因此，在所有病例中，获得了具有足够覆盖面积的支架生物瓣膜，组织损伤很小。阀门开启平稳，孔径比高。因此，这种新技术在构建具有足够瓣膜功能的健壮的、完全自体的支架生物瓣膜方面非常有效。(C) 2013 Wiley期刊公司
We designed a novel method for constructing an autologous heart valve with a stent, called a stent-biovalve. In constructing completely autologous heart valves, named biovalves, which used in-body tissue architecture technology, tissues for leaflets were formed via ingrowths into narrow apertures in the preparation molds, frequently leading to delayed or incomplete biovalve preparation. In this technique, self-expandable nitinol stents after everting were mounted on an acrylic column-shaped part and partially covered with an acrylic cylinder-shaped part with three slits. This assembled mold was placed into subcutaneous abdominal pouches in beagles or goats for 4 weeks. Upon removing the acrylic parts after harvesting and trimming of capsulated tissues, a tubular hollow structure with three pocket-flaps of membranous tissue rigidly fixed to the stent's outer surface was obtained. Then, the stent was turned inside out to the original form, thus moving the pocket-flaps from outside to the inside. Stent-biovalves with a sufficient coaptation area were thus obtained with little tissue damage in all cases. The valve opened smoothly, and high aperture ratio was noted. This novel technique was thus highly effective in constructing a robust, completely autologous stent-biovalve with adequate valve function. (C) 2013 Wiley Periodicals, Inc.