A Chinese 4-herb formula, Yiqi-Huoxue granule, alleviates H2O2-induced apoptosis by upregulating uncoupling protein 2 in H9c2 cells
A Chinese 4-herb formula, Yiqi-Huoxue granule, alleviates H2O2-induced apoptosis by upregulating uncoupling protein 2 in H9c2 cells
复制标题
中药四味方益气活血颗粒通过上调 H9c2 细胞中的解偶联蛋白 2 减轻 H2O2 诱导的细胞凋亡
DOI：
10.1016/j.phymed.2018.09.031
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发表时间：
2019-02-01
期刊：
影响因子：
7.9
通讯作者：
Han Lihua
中科院分区：
文献类型：
--
作者：
Wu Hong;Gao Haixia;Han Lihua
背景资料：虽然益气活血颗粒（YQHX）对缺血性心脏病患者的保护作用与减轻氧化应激损伤有关，但这些作用的机制仍不清楚。目的：我们的目的是研究YQHX治疗对过氧化氢（H2 O2）诱导的大鼠H9 c2细胞氧化应激的潜在保护作用。用YQHX处理H9 c2细胞16 h，然后暴露于200 μ M H2 O2 6 h。Hoechst 33，342染色和Annexin-V FITC/PI法检测H2 O2诱导的细胞凋亡。免疫印迹法检测解偶联蛋白2（UCP 2）、Bcl-2、Bax和caspase-3的表达。结果：H2 O2暴露后，H9 c2细胞氧化应激明显增强，细胞凋亡和ROS生成增加，UCP 2表达降低。0.75和1.5mg/ml的益气活血方能显著降低Bax和caspase-3的表达，而增加Bcl-2和UCP 2的蛋白表达。结论：总之，我们的研究表明，YQHX通过上调UCP 2的表达来减弱H2 O2诱导的H9 c2细胞凋亡，这表明YQHX是一种有前途的治疗I/R损伤介导的凋亡的方法。
Background: Although the protective effects of Yiqi-Huoxue granule (YQHX), a Chinese 4-herb formula, on patients with ischemic heart diseases are related to the attenuation of oxidative stress injury, the mechanism(s) underlying these actions remains poorly understood.Purpose: Our aim was to investigate the potential protective effects of YQHX treatment against oxidative stress induced by hydrogen peroxide (H2O2) in rat H9c2 cells.Methods: H9c2 cells were treated with YQHX for 16 h before exposed to 200 mu M H2O2 for 6 h. The apoptosis induced by H2O2 was measured using hoechst 33,342 staining and Annexin-V FITC/PI assay. The expression of uncoupling protein 2 (UCP2), Bcl-2, Bax, and caspase-3 were observed using western blot. The effects of UCP2 knockdown on cell apoptosis and intracellular ROS production were also investigated.Results: H2O2 exposure led to significant activation of oxidative stress followed by increased apoptosis and ROS production, as well as decreased UCP2 expression in H9c2 cells. YQHX treatment at the concentration of 0.75 and 1.5 mg/ml remarkably reduced the expression of Bax and caspase-3, whereas increased the protein expression of Bcl-2 and UCP2. These changes were attenuated by transgenic knockdown of UCP2 with Lenti-shUCP2 vector.Conclusions: Taken together, our study demonstrated that YQHX attenuates H2O2-induced apoptosis by upregulating UCP2 expression in H9c2 Cells, suggesting that YQHX is a promising therapeutic approach for the treatment of I/R injury-mediated apoptosis.