Epitope-Specific Anti-hCG Vaccines on a Virus Like Particle Platform.
Epitope-Specific Anti-hCG Vaccines on a Virus Like Particle Platform.
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DOI：
10.1371/journal.pone.0141407
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Peabody DS
中科院分区：
文献类型：
--
作者：
Caldeira J;Bustos J;Peabody J;Chackerian B;Peabody DS
以人绒毛膜促性腺激素为靶点的避孕疫苗的可能性早已被认识到，但从未完全实现。在这里，我们描述了一个表位特异性的方法的基础上的免疫原性展示的hCG衍生肽的病毒样颗粒的RNA噬菌体。构建了许多重组VLP，每个VLP展示不同的hCG衍生肽。一些来自激素的无序C末端尾部，另一个来自内环，还有一个是通过对hCG中和抗体靶点的亲和选择产生的表位模拟物。用一些VLP免疫小鼠产生抗血清，其结合激素并中和hCG生物活性。
The possibility of a contraceptive vaccine targeting human chorionic gonadotropin has long been recognized, but never fully realized. Here we describe an epitope-specific approach based on immunogenic display of hCG-derived peptides on virus-like particles of RNA bacteriophage. A number of recombinant VLPs were constructed, each displaying a different hCG-derived peptide. Some were taken from the disordered C-terminal tail of the hormone, another came from an internal loop, and yet another was an epitope mimic produced by affinity-selection on an hCG-neutralizing antibody target. Immunization of mice with some VLPs yielded antisera that bound the hormone and neutralized hCG biological activity.