Effects of l-theanine on glutamine metabolism in enterotoxigenic Escherichia coli (E44813)-stressed and non-stressed rats
Effects of l-theanine on glutamine metabolism in enterotoxigenic Escherichia coli (E44813)-stressed and non-stressed rats
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DOI：
10.1016/j.jff.2019.103670
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发表时间：
2020
影响因子：
5.6
通讯作者：
An Liu;Zhihua Gong;Ling Lin;Wei Xu;Tuo Zhang;Sheng Zhang;Yinhua Li;Jinhua Chen;W. Xiao
中科院分区：
文献类型：
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作者：
An Liu;Zhihua Gong;Ling Lin;Wei Xu;Tuo Zhang;Sheng Zhang;Yinhua Li;Jinhua Chen;W. Xiao
L-茶氨酸是一种非蛋白质衍生氨基酸，广泛用作食品中的功能性成分。在这里，我们评估了l-茶氨酸对健康大鼠和应激大鼠模型中谷氨酰胺代谢的体内影响，该模型是通过用产肠毒素大肠杆菌（E44813）感染大鼠而建立的。结果表明，l-茶氨酸显着增强健康大鼠谷氨酰胺的合成并稳定肠道。在E44813应激大鼠中，l-茶氨酸可以替代l-谷氨酰胺，从而通过减少炎症因子的表达、增强抗氧化酶的活性以及通过调节谷氨酰胺合成来调节谷氨酰胺代谢来缓解肠道应激。 l-茶氨酸和l-谷氨酰胺联合治疗在增强抗氧化酶活性、减少炎症因子表达、增加肠绒毛高度和隐窝深度方面优于单独用l-谷氨酰胺治疗。
l-theanine, a non-protein-derived amino acid, is widely used as a functional ingredient in foods. Here, we evaluated thein vivoeffects ofl-theanine on glutamine metabolism in healthy rats and in a stressed rat model, which was established by infecting rats with enterotoxigenicEscherichia coli(E44813). The results showed thatl-theanine significantly enhanced the synthesis of glutamine and stabilized the intestinal tract in healthy rats. In E44813-stressed rats,l-theanine could replacel-glutamine, thereby alleviating intestinal stress by reducing the expression of inflammatory factors, enhancing the activities of antioxidant enzymes, and regulating glutamine metabolism by modulating its synthesis. Treatment with a combination ofl-theanine andl-glutamine was superior to treatment withl-glutamine alone in enhancing the activity of antioxidant enzymes, reducing the expression of inflammatory factors, and increasing the intestinal villi height and crypt depth.