HIPPOCAMPAL PYRAMIDAL CELLS EXCITE INHIBITORY NEURONS THROUGH A SINGLE RELEASE SITE
HIPPOCAMPAL PYRAMIDAL CELLS EXCITE INHIBITORY NEURONS THROUGH A SINGLE RELEASE SITE
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DOI：
10.1038/366683a0
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发表时间：
1993-12-16
期刊：
影响因子：
64.8
通讯作者：
FREUND, TF
中科院分区：
文献类型：
--
作者：
GULYAS, AI;MILES, R;FREUND, TF
从形态上讲，突触由含有囊泡的突触前释放部位、具有膜特化的突触后成分以及它们之间的突触裂隙组成。释放部位的数量决定了神经元2-4之间突触传递的特性。虽然已经研究了皮质神经元之间的兴奋性相互作用5-9，但释放部位的数量仍然未知。我们现在已经记录了单个锥体细胞在海马区中间神经元中诱发的兴奋性突触后电位，并使用生物细胞素注射对两个细胞进行了可视化。光镜和电子显微镜显示兴奋性突触后电位是由单个突触介导的。我们还重建了单个锥体细胞的整个轴突树枝，填充在体内，切片上进行小白蛋白反染，选择性地标记篮子和轴突细胞10，11。锥体细胞和含有小白蛋白的神经元之间的单一突触接触占主导地位(>；80%)，为海马区网络的高度收敛和发散提供了证据12。
MORPHOLOGICALLY a synapse consists of a presynaptic release site containing vesicles, a postsynaptic element with membrane specialization, and a synaptic cleft between them1. The number of release sites shapes the properties of synaptic transmission between neurons2-4. Although excitatory interactions between cortical neurons have been examined5-9, the number of release sites remains unknown. We have now recorded excitatory postsynaptic potentials evoked by single pyramidal cells in hippocampal interneurons and visualized both cells using biocytin injections. Light and electron microscopy showed that excitatory postsynaptic potentials were mediated by a single synapse. We also reconstructed the entire axon arborization of single pyramidal cells, filled in vivo, in sections counterstained for parvalbumin, which selectively marks basket and axo-axonic cells10,11. Single synaptic contacts between pyramidal cells and parvalbumin-containing neurons were dominant (>80%), providing evidence for high convergence and divergence in hippocampal networks12.