Constitutive activity of membrane-inserted protein kinase C.
Constitutive activity of membrane-inserted protein kinase C.
复制标题
膜插入蛋白激酶 C 的组成活性。
DOI：
10.1016/s0006-291x(88)80719-8
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发表时间：
1988
影响因子：
3.1
通讯作者：
Nelsestuen,GL
中科院分区：
文献类型：
--
作者：
Bazzi,MD;Nelsestuen,GL
纯化的蛋白激酶C(PKC)与磷脂微囊孵育产生两组膜结合的PKC：一组被钙离子螯合解离，另一组不解离。第二个群体似乎被插入到细胞膜中。膜内PKC的活性不依赖于钙离子，对佛波醇酯仅有一定的敏感性。插入是由高钙浓度或佛波酯加低钙引起的。这些条件与激活PKC所需的条件相关；插入细胞膜可能是PKC激活的主要机制。PKC可能是一种长期的细胞调节剂，当第二信使钙和二酰甘油出现时，它就会被插入到膜中，当第二信使被移除时，它仍然处于活跃的膜结合状态。
Incubation of purified protein kinase C (PKC) with phospholipid vesicles produced two populations of membrane-bound PKC: one population was dissociated by calcium chelation and the other was not. The second population appeared to be inserted into the membrane. The activity of membrane-inserted PKC was Ca2+-independent and was only modestly sensitive to phorbol esters. Insertion was caused by high calcium concentrations or by phorbol esters plus low calcium. These conditions correlated with those needed to activate PKC; insertion into the membrane may be a primary mechanism of PKC activation. PKC may be a long-term cell regulator which becomes inserted into the membrane upon appearance of the second messengers, calcium and diacylglycerol, and remains in an active membrane-bound state when the second messengers have been removed.