ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF
ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF
复制标题
DOI：
10.1016/s0092-8674(00)80796-5
复制
发表时间：
1999-06-25
期刊：
影响因子：
64.5
通讯作者：
Wu, C
中科院分区：
文献类型：
--
作者：
Hamiche, A;Sandaltzopoulos, R;Wu, C
Drosophila BNF是一种ATP依赖性染色质重塑复合物，含有ATP酶SW 12/SNF 2家族成员ISWI。我们证明，BMPF催化的hsp 70启动子DNA上重建的单核细胞体的双向再分布。在有BNF存在的情况下，核小体在几分钟内从一系列可能的位置中选择一个占优势的位置。运动顺式发生，没有转移到竞争的DNA。通过fro III消化捕获的迁移中间体揭示了几个碱基对增量的渐进核小体运动。在此过程中，所有四种核心组蛋白均定量保留，表明组蛋白八聚体的总体完整性得以保持。我们认为，BMPF重塑核小体通过瞬时降低活化能的组蛋白八聚体的短程滑动。
Drosophila NURF is an ATP-dependent chromatin remodeling complex that contains ISWI, a member of the SW12/SNF2 family of ATPases. We demonstrate that NURF catalyzes the bidirectional redistribution of mononucleosomes reconstituted on hsp70 promoter DNA. In the presence of NURF, nucleosomes adopt one predominant position from an ensemble of possible locations within minutes. Movements occur in cis, with no transfer to competing DNA. Migrating intermediates trapped by fro III digestion reveal progressive nucleosome motion in increments of several base pairs. All four core histones are retained quantitatively during this process, indicating that the general integrity of the histone octamer is maintained. We suggest that NURF remodels nucleosomes by transiently decreasing the activation energy for short-range sliding of the histone octamer.