The taste bud and its innervation in the rat as studied by immunohistochemistry for PGP 9.5.
The taste bud and its innervation in the rat as studied by immunohistochemistry for PGP 9.5.
复制标题
通过 PGP 9.5 的免疫组织化学研究大鼠的味蕾及其神经支配。
DOI：
10.1679/aohc.59.357
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发表时间：
1996
影响因子：
--
通讯作者：
Sumio Yoshie
中科院分区：
文献类型：
--
作者：
H. Kanazawa;Sumio Yoshie
用PGP-9.5免疫组织化学方法在光镜和电子显微镜水平观察大鼠瓦状乳头区的味蕾，并进行常规的透射电子显微镜观察。PGP 9.5是动脉瘤旁的标志蛋白，定位于III型或味觉细胞的胞浆中。神经纤维中有更强的免疫反应，但部分神经纤维仍未被染色。免疫组织化学染色和常规电子显微镜观察发现，神经纤维形成一个粗大的上皮下神经丛，通过基底膜向上发出分支。在味蕾的底部，这些纤维形成了一个迄今未知的致密而精致的网状结构的宝石内丛。反过来，这个神经丛在味蕾细胞之间延伸串珠纤维，形成与III型细胞的突触接触。光镜下可见部分免疫反应神经纤维到达味孔；电子显微镜检查不能证实其直接暴露于味孔间隙。这项研究还研究了大鼠味蕾的精细结构，特别是III型细胞及其与神经纤维的突触，因为大鼠最近在这一领域的研究相当少。
The taste bud in the rat vallate papillae was observed by immunohistochemistry for PGP 9.5 at the light and electron microscope levels; routine transmission electron microscopy was also performed. Immunoreactivity for PGP 9.5, a marker protein of paraneurons, was localized in the cytoplasm of the Type III or gustatory cells. More intensive immunoreactivity occurred in the nerve fibers, though a part of the nerve fibers remained unstained. The nerve fibers as detected by the immunostaining and by routine electron microscopy formed a coarse subepithelial plexus which issued branches upwards through the basal lamina. In the basal portion of the taste bud, these fibers formed a hitherto unknown intragemmal plexus of dense and delicate meshwork. This plexus, in turn, extended beaded fibers between the taste bud cells, forming synaptic contacts with Type III cells. Some of the immunoreactive nerve fibers were confirmed to reach the taste pore by light microscopy; electron-microscopic examination could not demonstrate their directly being exposed to the space of the taste pore. This study also deals with the fine structure of the rat taste bud with special reference to the Type III cells and their synapses with nerve fibers, as the rat has recently been rather seldom used in this field of study.