Vapor-liquid-solid grown silica nanowire based electrochemical glucose biosensor.
Vapor-liquid-solid grown silica nanowire based electrochemical glucose biosensor.
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DOI：
10.1039/c0an00977f
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
E. Murphy-Pérez;S. Arya;S. Bhansali
中科院分区：
文献类型：
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作者：
E. Murphy-Pérez;S. Arya;S. Bhansali
采用气相-液相-固相（VLS）法在金电极上制备了二氧化硅纳米线（SiO2），并将其用于葡萄糖氧化酶（GOx）的共价固定。通过3-氨基丙基三乙氧基硅烷（APTES）改性和N-乙基-N '-（3-二甲基氨基丙基）碳二亚胺和N-羟基琥珀酰亚胺化学实现共价结合。用扫描电镜、透射电镜和循环伏安法对SiO（2）NW和GOx/APTES/SiO（2）NW/Au生物电极进行了表征。电化学研究表明，SiO（2）NW增加了有效的电活性表面积，从而导致更高的载酶量。响应特性显示，在25-300 mg dl（-1）的目标范围内呈线性，检测限为11 mg dl（-1），灵敏度为0.463 µA（mg dl（-1））（-1），回归系数为0.992。
Vapor-liquid-solid (VLS) grown silica nanowires (SiO(2)NWs) have been deposited electrophoretically on a gold electrode and utilized for covalent immobilization of glucose oxidase (GOx). Covalent binding has been achieved via 3-aminopropyltriethoxysilane (APTES) modification and N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. Scanning electron microscopy, transmission electron microscopy and cyclic voltammetry techniques have been used to characterize SiO(2)NW and GOx/APTES/SiO(2)NW/Au bioelectrode. Electrochemical studies reveal that SiO(2)NW increases the effective electro-active surface area thus resulting in higher loading of enzyme. Response characteristics show linearity in the range of interest 25-300 mg dl(-1), with a detection limit of 11 mg dl(-1), sensitivity: 0.463 µA (mg dl(-1))(-1) and regression coefficient of 0.992.