Optical control of excitation waves in cardiac tissue.
Optical control of excitation waves in cardiac tissue.
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DOI：
10.1038/nphoton.2015.196
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发表时间：
2015-12
期刊：
影响因子：
35
通讯作者：
Bub G
中科院分区：
文献类型：
--
作者：
Burton RA;Klimas A;Ambrosi CM;Tomek J;Corbett A;Entcheva E;Bub G
在自然界中，宏观激发波存在于各种各样的环境中，包括化学反应，金属生锈，酵母，变形虫以及心脏和大脑。在活的生物组织的情况下，由这些激励波形成的时空图案在健康和患病状态下是不同的。目前用于波调制的电学和药理学方法缺乏控制这些模式所需的时空精度。光学方法有可能克服这些限制，但迄今为止仅在简单系统中得到证实，例如Belousov-Zhabotinsky化学反应。在这里，我们将联合收割机无染料光学成像与光遗传学驱动相结合，实现心脏兴奋波的动态控制。用图案化的光照明被证明可以光学控制心脏组织中这种波的方向、速度和螺旋手性。这种全光学方法为研究和控制复杂生物可激发系统中的图案形成提供了一个新的实验平台。
In nature, macroscopic excitation waves are found in a diverse range of settings including chemical reactions, metal rust, yeast, amoeba and the heart and brain. In the case of living biological tissue, the spatiotemporal patterns formed by these excitation waves are different in healthy and diseased states. Current electrical and pharmacological methods for wave modulation lack the spatiotemporal precision needed to control these patterns. Optical methods have the potential to overcome these limitations, but to date have only been demonstrated in simple systems, such as the Belousov–Zhabotinsky chemical reaction. Here, we combine dye-free optical imaging with optogenetic actuation to achieve dynamic control of cardiac excitation waves. Illumination with patterned light is demonstrated to optically control the direction, speed and spiral chirality of such waves in cardiac tissue. This all-optical approach offers a new experimental platform for the study and control of pattern formation in complex biological excitable systems.