Application of High-Throughput Sequencing in Medicinal Plant Transcriptome Studies
Application of High-Throughput Sequencing in Medicinal Plant Transcriptome Studies
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DOI：
10.1002/ddr.21041
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发表时间：
2012-12-01
影响因子：
3.8
通讯作者：
Liu, Ming
中科院分区：
文献类型：
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作者：
Hao, Da-Cheng;Chen, Shi-Lin;Liu, Ming
药用植物转录组分析的两种替代方法，RNA测序（RNA-seq）和数字基因表达（DGE），是基于序列的，并且由于高通量测序技术的快速发展而变得越来越受欢迎。在高通量测序技术中，454焦磷酸测序和Illumina测序平台是最早商业化并建立的，因此广泛应用于药用植物转录组研究的各个领域。RNA-seq技术可以促进药用植物代谢途径的分析和分子标记的挖掘，为药用植物的分子育种奠定基础。DGE为药用植物的生化机制提供了新的见解。本文综述了高通量测序在药物发现、药物开发和植物天然产物大规模生产中的潜力，并展望了其在药用植物转录组研究中的未来发展和应用。药物开发研究73：487-498，2012年。(C)2012 Wiley Periodicals，Inc.
Two alternative approaches to medicinal plant transcriptome analysis, RNA sequencing (RNA-seq) and digital gene expression (DGE), are sequence based and have become increasingly popular due to rapid developments in high-throughput sequencing technologies. Among the high-throughput sequencing techniques, the 454 pyrosequencing and Illumina sequencing platforms are the first to be commercially available and established and thus widely used in various fields of medicinal plant transcriptome research. Metabolic pathway analysis of medicinal plants and molecular marker mining for molecular breeding can be facilitated and accelerated by RNA-seq. DGE provides novel insights into biochemical mechanisms in medicinal plants. This review illustrates the potential of high-throughput sequencing in drug discovery, drug development, and large-scale production of plant natural products, and envisions its future development and applications in medicinal plant transcriptome studies. Drug Dev Res 73 : 487-498, 2012. (C) 2012 Wiley Periodicals, Inc.