Lipid Organization of the Plasma Membrane
Lipid Organization of the Plasma Membrane
复制标题
DOI：
10.1021/ja507832e
复制
发表时间：
2014-10-15
影响因子：
15
通讯作者：
Marrink, Siewert J.
中科院分区：
文献类型：
--
作者：
Ingolfsson, Helgi I.;Melo, Manuel N.;Marrink, Siewert J.
细胞膜的详细组织仍然相当难以捉摸。基于大规模分子动力学模拟，我们以前所未有的复杂性水平提供了质膜脂质组织的高分辨率视图。我们的质膜模型由63种不同的脂质组成，结合14种头群和11种不对称分布在两个小叶上的尾，非常接近于模拟理想的哺乳动物质膜。我们观察到外小叶中胆固醇的富集和不同脂质种类的一般非理想的侧向混合。具有液体有序特征的瞬态域在微秒时间尺度上形成并消失。这些结构域通过两个膜小叶偶联。在外层小叶中，观察到不同的由神经节苷脂组成的纳米结构域。磷酸肌苷在内小叶中优先聚集。我们的数据提供了一个关键的观点，脂质横向组织在生命的基本结构之一，细胞膜。
The detailed organization of cellular membranes remains rather elusive. Based on large-scale molecular dynamics simulations, we provide a high-resolution view of the lipid organization of a plasma membrane at an unprecedented level of complexity. Our plasma membrane model consists of 63 different lipid species, combining 14 types of headgroups and 11 types of tails asymmetrically distributed across the two leaflets, closely mimicking an idealized mammalian plasma membrane. We observe an enrichment of cholesterol in the outer leaflet and a general non-ideal lateral mixing of the different lipid species. Transient domains with liquid-ordered character form and disappear on the microsecond time scale. These domains are coupled across the two membrane leaflets. In the outer leaflet, distinct nanodomains consisting of gangliosides are observed. Phosphoinositides show preferential clustering in the inner leaflet. Our data provide a key view on the lateral organization of lipids in one of lifes fundamental structures, the cell membrane.