Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography
Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography
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DOI：
10.1021/acs.analchem.6b03293
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发表时间：
2017-01-17
影响因子：
7.4
通讯作者：
Laskin, Julia
中科院分区：
文献类型：
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作者：
Nguyen, Son N.;Liyu, Andrey V.;Laskin, Julia
将剪切力探针与纳米desi探针相结合，建立了纳米喷雾解吸电喷雾电离（nano-DESI）生物样品等距离模式质谱成像（MSI）的新方法。介绍了该系统的技术概念和基本仪器设置，以及系统的一般工作原理。由于探针和样品表面之间存在剪切力，共振振荡的机械阻尼通过计算机控制的闭环反馈回路实现了恒定距离成像模式。利用活枯草芽孢杆菌ATCC 49760菌落在琼脂平板上，证明了复杂生物样品同时化学和地形成像的能力。恒距模式纳米desi MSI能够成像许多代谢物，包括活细菌菌落表面的非核糖体肽（表面素、普司他丁和iturin），直径从10到13 mm不等，高度变化可达琼脂板上方0.8 mm。离子图像与地形图像的共配准提供了更高对比度的图像。基于这一努力，恒定模式纳米desi MSI被证明非常适合于在其原生状态下成像复杂地形的生物样品。
A new approach for constant-distance mode mass spectrometry imaging (MSI) of biological samples using nanospray desorption electrospray ionization (nano-DESI) was developed by integrating a shear-force probe with the nano-DESI probe. The technical concept and basic instrumental setup, as well as the general operation of the system are described. Mechanical dampening of resonant oscillations due to the presence of shear forces between the probe and the sample surface enabled the constant-distance imaging mode via a computer-controlled closed-feedback loop. The capability of simultaneous chemical and topographic imaging of complex biological samples is demonstrated using living Bacillus subtilis ATCC 49760 colonies on agar plates. The constant-distance mode nano-DESI MSI enabled imaging of many metabolites, including nonribosomal peptides (surfactin, plipastatin, and iturin) on the surface of living bacterial colonies, ranging in diameter from 10 to 13 mm, with height variations up to 0.8 mm above the agar plate. Co-registration of ion images to topographic images provided higher-contrast images. Based on this effort, constant-mode nano-DESI MSI proved to be ideally suited for imaging biological samples of complex topography in their native states.