Labeling of vagal motoneurons and central afferents after injection of cholera toxin B into the airway lumen.
Labeling of vagal motoneurons and central afferents after injection of cholera toxin B into the airway lumen.
复制标题
将霍乱毒素 B 注射到气道腔后迷走神经运动神经元和中枢传入神经的标记。
DOI：
10.1152/ajplung.2001.280.1.l152
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Velloff,CR
中科院分区：
文献类型：
--
作者：
PérezFontán,JJ;Velloff,CR
我们检验了将霍乱毒素（CTB）的B亚基应用于气道粘膜会在延髓内产生神经元体和感觉纤维标记的假设。使用125i -CTB作为示踪剂，我们首先证明了CTB通过气管上皮运输，但一旦进入气道壁，它仍然局限于上皮下间隙和固有层。尽管这些区域内的固有神经元很少，但腔内CTB在模棱两可核中标记约10-60个神经元/大鼠，而在迷走神经背运动核中标记的神经元数量较少。在孤立束核的联合核、内侧核和腹外侧核的感觉纤维末端也有明确的标记。大约50%和90%的经腔内CTB标记的神经元也分别通过向气管外膜和肺实质注射氟金进行标记。这些结果表明，大量的髓质迷走神经运动神经元支配气道上皮附近的靶标。这些神经元在解剖学上似乎与投射到气道壁或肺实质较深层的迷走神经运动神经元没有分离。
We tested the hypothesis that application of the subunit B of cholera toxin (CTB) to the airway mucosa would produce labeling of neuronal somata and sensory fibers in the medulla oblongata. Using125I-CTB as a tracer, we demonstrated first that CTB is transported across the tracheal epithelium, but once in the airway wall, it remains confined to the subepithelial space and lamina propria. Despite the rarity of intrinsic neurons in these areas, intraluminal CTB labeled ≈10–60 neurons/rat in the nucleus ambiguus and a smaller number of neurons in the dorsal motor nucleus of the vagus. Well-defined sensory fiber terminals were also labeled in the commissural, medial, and ventrolateral subnuclei of the nucleus of the tractus solitarius. Approximately 50 and 90% of the neurons labeled by intraluminal CTB were also labeled by injections of FluoroGold into the tracheal adventitia and lung parenchyma, respectively. These findings demonstrate that a substantial number of medullary vagal motoneurons innervate targets in the vicinity of the airway epithelium. These neurons do not appear to be segregated anatomically from vagal motoneurons that project to deeper layers of the airway wall or lung parenchyma.