Neutrophil functions in morbidly obese subjects
Neutrophil functions in morbidly obese subjects
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DOI：
10.1111/cei.12631
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发表时间：
2015-07-01
影响因子：
4.6
通讯作者：
Levy, R.
中科院分区：
文献类型：
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作者：
Brotfain, E.;Hadad, N.;Levy, R.
本研究旨在确定 18 名体重指数 (BMI) 范围在 35 至 69 kg/m(2) 之间的病态肥胖受试者的不同外周血中性粒细胞功能，并与年龄和性别匹配的瘦对照受试者平行。测定了肥胖受试者和相匹配的瘦对照者的外周血中性粒细胞功能。与瘦对照相比，肥胖受试者的中性粒细胞显示基础超氧化物（P < 00001）、甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）刺激的超氧化物（P < 00001）和调理酵母聚糖（OZ）刺激的超氧化物（P < 0045）的释放显着升高。有趣的是，肥胖受试者和对照组的中性粒细胞在佛波醇肉豆蔻酸酯乙酸酯 (PMA) 刺激的超氧化物产生方面没有差异。与瘦对照相比，肥胖受试者的中性粒细胞趋化性（P<00003）和随机性（P<00001）迁移也显着升高。肥胖受试者的吞噬作用、CD11b 表面表达和中性粒细胞粘附与瘦对照者没有显着差异。超氧化物产生和趋化活性升高，加上正常的吞噬作用和粘附作用，表明肥胖受试者的中性粒细胞已准备好并具有抵抗感染的能力。然而，处于启动状态的中性粒细胞可能参与肥胖相关疾病的发病机制。
The present study aimed to determine different peripheral blood neutrophil functions in 18 morbidly obese subjects with body mass index (BMI) ranging between 35 and 69 kg/m(2) in parallel with age- and gender-matched lean controls. Peripheral blood neutrophil functions of obese subjects and matched lean controls were determined. Neutrophils of obese subjects showed significant elevation of the release of basal superoxides (P < 00001), formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated superoxides (P < 00001) and opsonized zymosan (OZ)-stimulated superoxides (P < 0045) compared with lean controls. Interestingly, there were no differences in phorbol myristate acetate (PMA)-stimulated superoxide production by neutrophils of the obese subjects and controls. There was also a significant elevation of chemotactic (P < 00003) and random (P < 00001) migration of neutrophils from obese subjects compared with lean controls. Phagocytosis, CD11b surface expression and adherence of neutrophils from obese subjects were not significantly different from those of the lean controls. The elevated superoxide production and chemotactic activity, together with the normal phagocytosis and adherence, suggest that neutrophils from obese subjects are primed and have the capability to combat infections. However, neutrophils in the priming state may participate in the pathogenesis of obesity-related diseases.