Aphid vector population density determines the emergence of necrogenic satellite RNAs in populations of cucumber mosaic virus
Aphid vector population density determines the emergence of necrogenic satellite RNAs in populations of cucumber mosaic virus
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DOI：
10.1099/jgv.0.000435
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发表时间：
2016-06-01
影响因子：
3.8
通讯作者：
Garcia-Arenal, Fernando
中科院分区：
文献类型：
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作者：
Betancourt, Monica;Fraile, Aurora;Garcia-Arenal, Fernando
黄瓜花叶病毒（CMV）的卫星RNA（N-satRNA）在番茄植株中诱导系统性坏死，在受感染的寄主中大量繁殖，同时严重抑制CMV的积累，从而降低蚜虫的传播效率。由于N-satRNA被传递到CMV颗粒中，因此N-satRNA出现的条件并不明显。模型分析与现实的参数值预测，N-satRNA将入侵CMV种群只有当传输率高。在这里，我们通过在低或高蚜虫密度（2或8只蚜虫/植物）下传代CMV或CMV+ N-satRNA来实验性地测试该假设。正如预测的那样，N-satRNA的出现需要高蚜虫密度。结果表明，在低蚜虫密度下，由于传播过程中的群体瓶颈导致的随机效应主导了CMV/CMV+ N-satRNA的流行动态。结果表明，保持蚜虫种群在低密度将防止出现高毒力CMV+ N-satRNA分离物。
The satellite RNAs of cucumber mosaic virus (CMV) that induce systemic necrosis in tomato plants (N-satRNA) multiply to high levels in the infected host while severely depressing CMV accumulation and, hence, its aphid transmission efficiency. As N-satRNAs are transmitted into CMV particles, the conditions for N-satRNA emergence are not obvious. Model analyses with realistic parameter values have predicted that N-satRNAs would invade CMV populations only when transmission rates are high. Here, we tested this hypothesis experimentally by passaging CMV or CMV+N-satRNAs at low or high aphid densities (2 or 8 aphids/plant). As predicted, high aphid densities were required for N-satRNA emergence. The results showed that at low aphid densities, random effects due to population bottlenecks during transmission dominate the epidemiological dynamics of CMV/CMV+ N-satRNA. The results suggest that maintaining aphid populations at low density will prevent the emergence of highly virulent CMV+ N-satRNA isolates.