In silico and in vitro analysis of small breast epithelial mucin as a marker for bone marrow micrometastasis in breast cancer
In silico and in vitro analysis of small breast epithelial mucin as a marker for bone marrow micrometastasis in breast cancer
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DOI：
10.1007/978-0-387-69080-3_31
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Aparicio, Luis Anton
中科院分区：
文献类型：
--
作者：
Ayerbes, Manuel Valladares;Diaz-Prado, Silvia;Aparicio, Luis Anton
与恶性表型相关的分子特征将有助于检测微转移癌细胞。小乳房上皮粘蛋白(SBEM)基因被预测为编码一种低分子糖蛋白。为了评价其作为乳腺癌患者骨髓微转移标志物的潜在作用，我们对SBEM基因的电子和体外表达谱进行了研究。在SAGE解剖查看器上显示和计数从正常组织和肿瘤组织和细胞系(CL)获得的数字SBEM表达文库。获得了用于检测播散性BC细胞的常用靶点--细胞角蛋白-19和乳头球蛋白(HMAM)的图谱，并与SBEM数据进行了比较。采用RT-PCR方法检测乳腺癌、造血癌CL和正常骨髓中SBEM和HMAM的表达。生物信息学工具被用来进一步了解SBEM在正常乳腺和BC中的生物学作用。用双向显示技术鉴定乳腺文库中与SBEM相关的表达模式的基因。在40个文库(21个BC；8个非癌乳腺组织)中检测到SBEM标签。在15/21个BC文库和7/8个非肿瘤乳腺组织中有中高表达。SBEM标签计数与ERBB2(0.662)、HMAM(0.409)和RRM2(-0.379)相关。以RT-PCR为基础的SBEM mRNA检测模型系统具有较高的敏感性和特异性，可用于检测分离的肿瘤细胞。我们的结果表明，SBEM mRNA可能是靶向BC微转移的重要标记物。
Molecular signatures associated with malignant phenotype would be useful for detection of micrometastatic carcinoma cells. The small breast epithelial mucin (SBEM) gene is predicted to code for a low molecular weight glycoprotein. To evaluate its potential role as a marker for bone marrow (BM) micrometastasis in breast cancer (BC) patients, we have studied in silico and in vitro expression profiles of SBEM gene. Digital SBEM expression in libraries obtained from normal and neoplastic tissues and cell -lines (CL) were displayed and counted on the SAGE Anatomic Viewer. Profiles for cytokeratin-19 and mammaglobin (hMAM), commonly targets used for detection of disseminated BC cells were obtained and compared with SBEM data. Human breast and haematopoietic cancer CL and normal BM were examined by RT-PCR for SBEM and hMAM. Bioinformatics tools were used to gain further insights about the biological role of SBEM in normal breast and BC. Genes with expression patterns in breast libraries correlating with SBEM were identified using two-dimensional display. SBEM tag was detected in 40 libraries (21 BC; 8 non-cancerous breast tissues). Intermediate to high expression was found on 15/21 BC libraries and 7/8 non-tumor breast tissue. SBEM tag count was correlated with ERBB2 (0.662), hMAM (0.409), and RRM2 (-0.379). A model system based on RT-PCR for SBEM mRNA was highly sensitive and specific in order to detect isolated tumor cells. Our results demonstrate that SBEM mRNA may be an imp ortant marker for targeting BC micrometastasis.